Contact guidance diversity in rotationally aligned collagen matrices

dc.contributor.author Nuhn, Jacob
dc.contributor.author Perez, Anai
dc.contributor.author Schneider, Ian
dc.contributor.department Genetics, Development and Cell Biology
dc.contributor.department Chemical and Biological Engineering
dc.date 2019-06-03T07:07:50.000
dc.date.accessioned 2020-06-30T01:09:49Z
dc.date.available 2020-06-30T01:09:49Z
dc.date.copyright Sun Jan 01 00:00:00 UTC 2017
dc.date.embargo 2019-11-28
dc.date.issued 2018-01-15
dc.description.abstract <p>Cancer cell metastasis is responsible for approximately 90% of deaths related to cancer. The migration of cancer cells away from the primary tumor and into healthy tissue is driven in part by contact guidance, or directed migration in response to aligned extracellular matrix. While contact guidance has been a focus of many studies, much of this research has explored environments that present 2D contact guidance structures. Contact guidance environments in 3D more closely resemble in vivo conditions and model cell-ECM interactions better than 2D environments. While most cells engage in directed migration on potent 2D contact guidance cues, there is diversity in response to contact guidance cues based on whether the cell migrates with a mesenchymal or amoeboid migration mode. In this paper, rotational alignment of collagen gels was used to study the differences in contact guidance between MDA-MB-231 (mesenchymal) and MTLn3 (amoeboid) cells. MDA-MB-231 cells migrate with high directional fidelity in aligned collagen gels, while MTLn3 cells show no directional migration. The collagen stiffness was increased through glycation, resulting in decreased MDA-MB-231 directionality in aligned collagen gels. Interestingly, partial inhibition of cell contractility dramatically decreased directionality in MDA-MB-231 cells. The directionality of MDA-MB-231 cells was most sensitive to ROCK inhibition, but unlike in 2D contact guidance environments, cell directionality and speed are more tightly coupled. Modulation of the contractile apparatus appears to more potently affect contact guidance than modulation of extracellular mechanical properties of the contact guidance cue.</p>
dc.description.comments <p>This is a manuscript of an article published as Nuhn, Jacob AM, Anai M. Perez, and Ian C. Schneider. "Contact guidance diversity in rotationally aligned collagen matrices." <em>Acta Biomaterialia</em> 66 (2018): 248-257. DOI: <a href="https://dx.doi.org/10.1016/j.actbio.2017.11.039" target="_blank">10.1016/j.actbio.2017.11.039</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/cbe_pubs/346/
dc.identifier.articleid 1346
dc.identifier.contextkey 13052065
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath cbe_pubs/346
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/13447
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/cbe_pubs/346/2018_Schneider_ContactGuidanceDiversity.pdf|||Fri Jan 14 23:42:30 UTC 2022
dc.source.uri 10.1016/j.actbio.2017.11.039
dc.subject.disciplines Biomaterials
dc.subject.disciplines Biomedical Engineering and Bioengineering
dc.subject.keywords Directed motility
dc.subject.keywords Three-dimensional
dc.subject.keywords Acupuncture
dc.subject.keywords Glycation
dc.subject.keywords Stiffness
dc.subject.keywords Confocal reflectance microscopy
dc.subject.keywords Breast cancer
dc.subject.keywords Orientation
dc.subject.keywords Blebbistatin
dc.subject.keywords Y-27632
dc.title Contact guidance diversity in rotationally aligned collagen matrices
dc.type article
dc.type.genre article
dspace.entity.type Publication
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relation.isOrgUnitOfPublication 9e603b30-6443-4b8e-aff5-57de4a7e4cb2
relation.isOrgUnitOfPublication 86545861-382c-4c15-8c52-eb8e9afe6b75
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