Studies of integrins' lateral dynamics at a single particle level

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dc.contributor.advisor Emily A. Smith
dc.contributor.author Mainali, Dipak
dc.contributor.department Chemistry
dc.date 2018-08-11T08:41:51.000
dc.date.accessioned 2020-06-30T02:52:57Z
dc.date.available 2020-06-30T02:52:57Z
dc.date.copyright Wed Jan 01 00:00:00 UTC 2014
dc.date.embargo 2015-03-01
dc.date.issued 2014-01-01
dc.description.abstract <p>One of the important factors in controlling the dynamics, structure, and functioning of the cell membrane is the lateral diffusion of membrane proteins and lipids. Integrins are one of the ubiquitous transmembrane proteins that are vital for numerous cellular functions. Integrins' lateral mobility and rearrangement is essential for integrins primary function of cell-extracellular matrix adhesion, cell-cell adhesion, and signaling. Lateral movement allows integrins to associate with extracellular ligand and other cytoplasmic and membrane proteins to incite biological activity such as signaling and downstream effects. This thesis describes the role of both downstream and upstream effectors, such as extracellular ligand, selected cytoplasmic and membrane proteins in altering integrins' lateral dynamics in the cell membrane. Integrins' lateral mobility is studied using two popular fluorescence techniques: single particle tracking (SPT) with ligand-functionalized quantum dots (QDs) and fluorescence recovery after photobleaching (FRAP) with integrin-Venus fusion proteins. The molecular biology technique called RNA interference (RNAi) is used to selectively reduce the expression of a protein of interest in the cell. The results prove that the ligand binding affinity, selected cytoplasmic and membrane proteins do affect lateral mobility of a subset of integrins.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/etd/13901/
dc.identifier.articleid 4908
dc.identifier.contextkey 6199616
dc.identifier.doi https://doi.org/10.31274/etd-180810-2613
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath etd/13901
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/28088
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/etd/13901/Mainali_iastate_0097E_14300.pdf|||Fri Jan 14 20:03:55 UTC 2022
dc.subject.disciplines Biophysics
dc.subject.disciplines Chemistry
dc.subject.disciplines Molecular Biology
dc.subject.keywords fluorescence
dc.subject.keywords lateral diffusion
dc.subject.keywords near infrared
dc.subject.keywords quantum dots
dc.subject.keywords receptors
dc.subject.keywords RNA interference
dc.title Studies of integrins' lateral dynamics at a single particle level
dc.type article
dc.type.genre dissertation
dspace.entity.type Publication
relation.isOrgUnitOfPublication 42864f6e-7a3d-4be3-8b5a-0ae3c3830a11
thesis.degree.level dissertation
thesis.degree.name Doctor of Philosophy
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