Understanding the biological activity of Hypericum species through metabolomics studies

dc.contributor.advisor Eve S. Wurtele
dc.contributor.advisor George A. Kraus
dc.contributor.advisor Basil Nikolau
dc.contributor.author Hillwig, Matthew
dc.contributor.department Theses & dissertations (Interdisciplinary)
dc.date 2018-08-22T13:54:44.000
dc.date.accessioned 2020-06-30T07:47:08Z
dc.date.available 2020-06-30T07:47:08Z
dc.date.copyright Tue Jan 01 00:00:00 UTC 2008
dc.date.issued 2008-01-01
dc.description.abstract <p>The Hypericum genus contains plants which biosynthesize various polyketides with various known and unknown biological and medicinal activities. However, many Hypericum species have not been chemically or biochemically characterized. These species represent an opportunity for understanding chemical ecology and for new drug discovery research. Acyl-phloroglucinols are of particular interest since they are the most diverse class of specialized metabolites in Hypericum. We used analytical methods such as HPLC, LC-MS and 2-D NMR to screen Hypericum species for metabolite diversity. One species, H. gentianoides, was investigated further based on its historical use by Native Americans and its unique metabolite fingerprint. This species was found to contain a set of biochemically-related dimeric acyl-phloroglucinols. Furthermore, H. gentianoides extracts revealed these compounds induce various biological responses in mammalian cells including anti-inflammatory responses, TRPV1 pain receptor-dependent calcium influx, and inhibition of two lentiviruses (HIV and EIAV). Extracts were highly cytotoxic to HeLa cancer cells. Bioactivity-guided fractionation of extracts defines specific acyl-phloroglucinols associated with the bioactivities. We propose that the bioactivities of these biochemically related acyl-phloroglucinols represent a coordinated process that alters cellular processes in other organisms via redox changes and protein interactions.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/rtd/15775/
dc.identifier.articleid 16774
dc.identifier.contextkey 7043036
dc.identifier.doi https://doi.org/10.31274/rtd-180813-16978
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath rtd/15775
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/69440
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/rtd/15775/3337386.PDF|||Fri Jan 14 20:46:20 UTC 2022
dc.subject.disciplines Biochemistry
dc.subject.disciplines Plant Biology
dc.subject.keywords Genetics
dc.subject.keywords development and cell biology;Plant biology;
dc.title Understanding the biological activity of Hypericum species through metabolomics studies
dc.type article
dc.type.genre dissertation
dspace.entity.type Publication
thesis.degree.discipline Plant Physiology
thesis.degree.level dissertation
thesis.degree.name Doctor of Philosophy
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