Loss of Gq/11 Genes Does Not Abolish Melanopsin Phototransduction

dc.contributor.author Chew, Kylie
dc.contributor.author Trimarchi, Jeffrey
dc.contributor.author Schmidt, Tiffany
dc.contributor.author Rupp, Alan
dc.contributor.author Kofuji, Paulo
dc.contributor.author Trimarchi, Jeffrey
dc.contributor.department Genetics, Development and Cell Biology
dc.date 2018-02-18T04:45:29.000
dc.date.accessioned 2020-06-30T04:02:50Z
dc.date.available 2020-06-30T04:02:50Z
dc.date.copyright Wed Jan 01 00:00:00 UTC 2014
dc.date.issued 2014-01-01
dc.description.abstract <p>In mammals, a subset of retinal ganglion cells (RGCs) expresses the photopigment melanopsin, which renders them intrinsically photosensitive (ipRGCs). These ipRGCs mediate various non-image-forming visual functions such as circadian photoentrainment and the pupillary light reflex (PLR). Melanopsin phototransduction begins with activation of a heterotrimeric G protein of unknown identity. Several studies of melanopsin phototransduction have implicated a G-protein of the Gq/11 family, which consists of <em>Gna11</em>, <em>Gna14</em>, <em>Gnaq</em> and <em>Gna15</em>, in melanopsin-evoked depolarization. However, the exact identity of the Gq/11 gene involved in this process has remained elusive. Additionally, whether Gq/11 G-proteins are necessary for melanopsin phototransduction <em>in vivo</em> has not yet been examined. We show here that the majority of ipRGCs express both <em>Gna11</em> and <em>Gna14</em>, but neither <em>Gnaq</em> nor <em>Gna15</em>. Animals lacking the melanopsin protein have well-characterized deficits in the PLR and circadian behaviors, and we therefore examined these non-imaging forming visual functions in a variety of single and double mutants for <em>Gq/11</em> family members. All <em>Gq/11</em> mutant animals exhibited PLR and circadian behaviors indistinguishable from WT. In addition, we show persistence of ipRGC light-evoked responses in <em>Gna11−/−; Gna14−/−</em> retinas using multielectrode array recordings. These results demonstrate that Gq, G11, G14, or G15 alone or in combination are not necessary for melanopsin-based phototransduction, and suggest that ipRGCs may be able to utilize a Gq/11-independent phototransduction cascade <em>in vivo</em>.</p>
dc.description.comments <p>This article is from <em>PloS ONE </em>9 (2014): e98356, doi: <a href="http://dx.doi.org/10.1371/journal.pone.0098356">10.1371/journal.pone.0098356</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/gdcb_las_pubs/83/
dc.identifier.articleid 1088
dc.identifier.contextkey 9723909
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath gdcb_las_pubs/83
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/37996
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/gdcb_las_pubs/83/2014_Trimarchi_LossGenes.PDF|||Sat Jan 15 02:09:31 UTC 2022
dc.source.uri 10.1371/journal.pone.0098356
dc.subject.disciplines Cell Biology
dc.subject.disciplines Developmental Biology
dc.subject.disciplines Genetics
dc.subject.disciplines Zoology
dc.title Loss of Gq/11 Genes Does Not Abolish Melanopsin Phototransduction
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 6e9f21d0-82ad-4661-a8f7-7634953c036b
relation.isOrgUnitOfPublication 9e603b30-6443-4b8e-aff5-57de4a7e4cb2
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