A helitron-like transposon superfamily from lepidoptera disrupts (GAAA)(n) microsatellites and is responsible for flanking sequence similarity within a microsatellite family

dc.contributor.author Coates, Brad
dc.contributor.author Hellmich, Richard
dc.contributor.author Sumerford, Douglas
dc.contributor.author Hellmich, Richard
dc.contributor.author Lewis, Leslie
dc.contributor.department Entomology
dc.date 2018-02-14T11:11:23.000
dc.date.accessioned 2020-06-30T02:21:44Z
dc.date.available 2020-06-30T02:21:44Z
dc.date.embargo 2014-08-28
dc.date.issued 2010-03-01
dc.description.abstract <p>Transposable elements (TEs) are mobile DNA regions that alter host genome structure and gene expression. A novel 588 bp non-autonomous high copy number TE in the Ostrinia nubilalis genome has features in common with miniature inverted-repeat transposable elements (MITEs): high A + T content (62.3%), lack of internal protein coding sequence, and secondary structure consisting of subterminal inverted repeats (SIRs). The O. nubilalis TE has inserted at (GAAA)n microsatellite loci, and was named the microsatellite-associated interspersed nuclear element ( MINE-1). Non-autonomous MINE-1 superfamily members also were identified downstream of (GAAA)n microsatellites within Bombyx mori and Pectinophora gossypiella genomes. Of 316 (GAAA)n microsatellites from the B. mori whole genome sequence, 201 (63.6%) have associated autonomous or non-autonomous MINE-1 elements. Autonomous B. mori MINE-1s a encode a helicase and endonuclease domain RepHel-like protein (BMHELp1) indicating their classification as Helitron-like transposons and were renamed Helitron1_BM. Transposition of MINE-1 members in Lepidoptera has resulted in the disruption of (GAAA)n microsatellite loci, has impacted the application of microsatellite-based genetic markers, and suggests genome sequence that flanks TT/AA dinucleotides may be required for target site recognition by RepHel endonuclease domains.</p>
dc.description.comments <p>This article is from <em>Journal of Molecular Evolution</em>; 70 (2010); 275-288; doi: <a href="http://dx.doi.org/10.1007/s00239-010-9330-6" target="_blank">10.1007/s00239-010-9330-6</a></p>
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dc.identifier archive/lib.dr.iastate.edu/ent_pubs/120/
dc.identifier.articleid 1125
dc.identifier.contextkey 6048211
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath ent_pubs/120
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/23722
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/ent_pubs/120/2010_HellmichRL_Helitron_LikeTransposon.pdf|||Fri Jan 14 19:10:14 UTC 2022
dc.source.uri 10.1007/s00239-010-9330-6
dc.subject.disciplines Entomology
dc.subject.disciplines Plant Breeding and Genetics
dc.subject.keywords Microsatellite family
dc.subject.keywords Transposable element
dc.subject.keywords Mobile element
dc.title A helitron-like transposon superfamily from lepidoptera disrupts (GAAA)(n) microsatellites and is responsible for flanking sequence similarity within a microsatellite family
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 3cca44d8-e1df-437d-a95a-d8e38963d2c1
relation.isOrgUnitOfPublication f47c8cad-50be-4fb0-8870-902ff536748c
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