Morphological and functional studies of cells derived from post-natal neural stem cells

dc.contributor.advisor Srdija Jeftinija
dc.contributor.advisor Shang-Yi Lin Rowe, Eric
dc.contributor.department Biomedical Sciences 2018-08-25T00:04:30.000 2020-06-30T08:02:04Z 2020-06-30T08:02:04Z Sat Jan 01 00:00:00 UTC 2005 2005-01-01
dc.description.abstract <p>This dissertation investigates the morphological and functional characteristics of cells that have been differentiated from post-natal neural stem cells. The population of neural stem cells that were investigated in these experiments are oligodendrocytic precursor cells (OPC). Previous studies have shown that OPC that are sequentially exposed to bone morphogenetic protein and basic fibroblast growth factor convert into a cell that can give rise to oligodendrocytes, astrocytes, and neurons. However, none of these previous studies investigated the functional properties of the differentiated cells;The second chapter of this work describes both the morphological and functional characteristics of the differentiated cells. We show that a large percentage of these cells differentiate into functional neurons that have voltage gated calcium channels, ligand gated calcium channels, and are able to release glutamate in response to ATP (adenosine triphosphate) stimulation. Furthermore, we show that these differentiated neurons have morphological markers consistent with neuronal differentiation;The third chapter of this dissertation demonstrates that astrocytes influence receptor expression in the differentiated neurons. The receptor that was investigated was N-methyl-D-aspartate (NMDA) which is a subtype of glutamate receptor and has been shown to be important in many processes critical to neuronal function.</p>
dc.format.mimetype application/pdf
dc.identifier archive/
dc.identifier.articleid 2769
dc.identifier.contextkey 6105406
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath rtd/1770
dc.language.iso en
dc.source.bitstream archive/|||Fri Jan 14 21:27:45 UTC 2022
dc.subject.disciplines Neuroscience and Neurobiology
dc.subject.disciplines Neurosciences
dc.subject.keywords Neuroscience
dc.subject.keywords Biomedical sciences
dc.subject.keywords Veterinary anatomy
dc.title Morphological and functional studies of cells derived from post-natal neural stem cells
dc.type article
dc.type.genre dissertation
dspace.entity.type Publication
relation.isOrgUnitOfPublication 184db3f2-d93f-4571-8ad7-07c8a9e6a5c9 Neuroscience; Veterinary Anatomy; dissertation Doctor of Philosophy
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