A cyst nematode effector binds to diverse plant proteins, increases nematode susceptibility and affects root morphology

dc.contributor.author Pogorelko, Gennady
dc.contributor.author Baum, Thomas
dc.contributor.author Juvale, Parijat
dc.contributor.author Rutter, William
dc.contributor.author Hewezi, Tarek
dc.contributor.author Hussey, Richard
dc.contributor.author Davis, Eric
dc.contributor.author Mitchum, Melissa
dc.contributor.author Baum, Thomas
dc.contributor.department Plant Pathology and Microbiology
dc.contributor.department Plant Biology
dc.date 2018-02-18T18:56:20.000
dc.date.accessioned 2020-06-30T06:22:37Z
dc.date.available 2020-06-30T06:22:37Z
dc.date.copyright Thu Jan 01 00:00:00 UTC 2015
dc.date.issued 2016-08-01
dc.description.abstract <p>Cyst nematodes are plant-parasitic roundworms that are of significance in many cropping systems around the world. Cyst nematode infection is facilitated by effector proteins secreted from the nematode into the plant host. The cDNAs of the 25A01-like effector family are novel sequences that were isolated from the esophageal gland cells of the soybean cyst nematode (Heterodera glycines). To aid functional characterization we identified an orthologous member of this protein family (Hs25A01) from the closely related sugar beet cyst nematode H. schachtii, which infects Arabidopsis. Constitutive expression of the Hs25A01 CDS in Arabidopsis plants caused a small increase in root length accompanied by up to 22% increase in susceptibility to H. schachtii. A plant-expressed RNAi construct targeting Hs25A01 transcripts in invading nematodes significantly reduced host susceptibility to H. schachtii. These data document that Hs25A01 has physiological functions in planta and a role in cyst nematode parasitism. In vivo as well as in vitro binding assays confirmed specific interactions of Hs25A01 with an Arabidopsis F-box containing protein, a chalcone synthase, and the translation initiation factor eIF- 2 beta subunit (eIF-2bs), making these proteins likely candidates for being involved in the observed changes in plant growth and parasitism. A role of eIF- 2bs in mediating the Hs25A01 virulence function is further supported by the observation that two independent eIF-2bs Arabidopsis knock-out lines were significantly more susceptible to H. schachtii.</p>
dc.description.comments <p>This is the peer reviewed version of the following article: Pogorelko, Gennady, Parijat S. Juvale, William B. Rutter, Tarek Hewezi, Richard Hussey, Eric L. Davis, Melissa G. Mitchum, and Thomas J. Baum. "A cyst nematode effector binds to diverse plant proteins, increases nematode susceptibility and affects root morphology." <em>Molecular plant pathology</em> 17, no. 6 (2016): 832-844, which has been published in final form at DOI: <a href="http://dx.doi.org/10.1111/mpp.12330" target="_blank">10.1111/mpp.12330</a>. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/plantpath_pubs/174/
dc.identifier.articleid 1172
dc.identifier.contextkey 10542493
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath plantpath_pubs/174
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/57617
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/plantpath_pubs/174/2016_Baum_CystNematode.pdf|||Fri Jan 14 21:22:12 UTC 2022
dc.source.uri 10.1111/mpp.12330
dc.subject.disciplines Agricultural Science
dc.subject.disciplines Agriculture
dc.subject.disciplines Agronomy and Crop Sciences
dc.subject.disciplines Genetics and Genomics
dc.subject.disciplines Plant Pathology
dc.subject.keywords functional characterization
dc.subject.keywords Heterodera
dc.subject.keywords RNAi
dc.subject.keywords interaction
dc.subject.keywords translation
dc.subject.keywords mutant
dc.title A cyst nematode effector binds to diverse plant proteins, increases nematode susceptibility and affects root morphology
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication ea8d53ba-1a8f-4f5c-9b4a-1ecbd67e2f43
relation.isOrgUnitOfPublication a26b5928-54bb-4a0b-a973-95d649d1ad83
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