Genetic Variation Among Chicken Lines and Mammalian Species in Specific Genes

dc.contributor.author Zhou, H.
dc.contributor.author Liu, W.
dc.contributor.author Lamont, Susan
dc.contributor.author Lamont, Susan
dc.contributor.department Animal Science
dc.date 2021-02-05T18:10:21.000
dc.date.accessioned 2021-02-24T21:14:05Z
dc.date.available 2021-02-24T21:14:05Z
dc.date.copyright Mon Jan 01 00:00:00 UTC 2001
dc.date.issued 2001-03-01
dc.description.abstract <p>Thirteen gene-specific primer sets provided by the U.S. Poultry Genome Coordinators were used to investigate DNA polymorphisms between two highly inbred chicken lines of Leghorn and Fayoumi origin. Nucleotide and predicted amino acid sequences were then compared among these chicken lines and the Genbank sequences of chicken, mouse, and human. The following genes were selected as candidates for immune response or transcription activation: <em>B2M, DAD1, IAP1, IL2, IREB1, LAP18, MAFL, POU1F1, RREB1, TAD, TBP1, TCRG,</em> and <em>ZOV3</em>. Total cDNA was obtained from the spleens of Leghorn and Fayoumi lines by reverse transcriptase-polymerase chain reaction (PCR) and was used as a template to PCR-amplify gene-specific products. All primers except <em>POU1F1</em> and <em>TCRG</em> generated single PCR products of the predicted 325- to 667-bp size, confirming the efficacy of these gene-specific primers in the chicken. Three and seven of the 11 amplified gene fragments yielded line-specific nucleotide polymorphisms between the Leghorn and Fayoumi sequences and between the Leghorn and Genbank chicken sequences respectively. Similarities between inbred Leghorn and mammalian species were 36 to 86% for nucleotides and 25 to 96% for predicted amino acid sequence. The polymorphisms of some gene fragments between the Leghorn and Fayoumi lines will allow for investigation of associations of these genes with immune response and other biological traits.</p>
dc.description.comments <p>This article is published as Zhou, Huaijun, Wei Liu, and Susan J. Lamont. "Genetic variation among chicken lines and mammalian species in specific genes." <em>Poultry Science</em> 80, no. 3 (2001): 284-288. DOI: <a href="https://doi.org/10.1093/ps/80.3.284" target="_blank">10.1093/ps/80.3.284</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/ans_pubs/718/
dc.identifier.articleid 1715
dc.identifier.contextkey 21465977
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath ans_pubs/718
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/93473
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/ans_pubs/718/2001_LamontSusan_GeneticVariation.pdf|||Sat Jan 15 01:43:32 UTC 2022
dc.source.uri 10.1093/ps/80.3.284
dc.subject.disciplines Agriculture
dc.subject.disciplines Genetics
dc.subject.disciplines Molecular Biology
dc.subject.disciplines Poultry or Avian Science
dc.subject.keywords inbred chicken line
dc.subject.keywords immune response
dc.subject.keywords candidate gene
dc.subject.keywords nucleotide sequence
dc.subject.keywords amino acid sequence
dc.title Genetic Variation Among Chicken Lines and Mammalian Species in Specific Genes
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 5dee3d24-aa7a-4fe1-abf6-f0bb615bfe24
relation.isOrgUnitOfPublication 85ecce08-311a-441b-9c4d-ee2a3569506f
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