Vitamin A equivalence of the β-carotene in biofortified cassava in women

dc.contributor.advisor Wendy S. White Liu, Wenhong
dc.contributor.department Food Science and Human Nutrition 2018-08-11T15:25:03.000 2020-06-30T02:32:14Z 2020-06-30T02:32:14Z Thu Jan 01 00:00:00 UTC 2009 2013-06-05 2009-01-01
dc.description.abstract <p>Cassava is an important food energy source in tropical and subtropical regions. There have been successful efforts to increase the β-carotene content in cassava through biofortification. However, the bioefficacy of the β-carotene in the biofortified cassava in humans has not been studied. In the present study, we investigated the bioefficacy of the β-carotene in biofortified cassava in 9 healthy women ages 18-39 y. After eating conventional foods with low β-carotene and vitamin A contents for three days, the subjects were asked to ingest one of the three cassava porridges in random order. All of the porridge servings were about 200 g and contained 40 g cassava flour. The three porridges included biofortified cassava porridge (1097.5 μg β-carotene), white cassava porridge with vitamin A reference dose (285.6 μg retinol) and white cassava porridge with β-carotene reference dose (537.6 μg β-carotene). Blood samples were collected prior to ingestion of the test porridge and at 2, 3.5, 5, 6.5, 9 hours after ingestion. Plasma triacylglycerol rich lipoproteins (chylomicrons and large VLDL) were isolated and the retinyl palmitate contents were analyzed by HPLC-ECD. The mean amounts of retinyl palmitate appearing in the triacylglycerol-rich lipoprotein fraction in the entire plasma pool after ingestion of the biofortified cassava porridge and the white cassava porridge with the β-carotene reference dose were 1587.53 ± 285.27 and 914.35 ± 128.50 nmol. The vitamin A equivalence values of the β-carotene in these two porridges were 2.80 ± 1.77 to 1 and 2.11 ± 0.81 to 1 (by weight), respectively. These vitamin A equivalence values were not significantly different by t test. In our study population, the bioefficacy of the β-carotene in biofortified cassava was as good as that of a β-carotene supplement.</p>
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dc.identifier archive/
dc.identifier.articleid 1996
dc.identifier.contextkey 2807194
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath etd/10958
dc.language.iso en
dc.source.bitstream archive/|||Fri Jan 14 18:31:43 UTC 2022
dc.subject.disciplines Nutrition
dc.title Vitamin A equivalence of the β-carotene in biofortified cassava in women
dc.type article
dc.type.genre thesis
dspace.entity.type Publication
relation.isOrgUnitOfPublication 4b6428c6-1fda-4a40-b375-456d49d2fb80 thesis Master of Science
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