Reactivity of Porcine Epidemic Diarrhea Virus Structural Proteins to Antibodies against Porcine Enteric Coronaviruses: Diagnostic Implications Giménez-Lirola, Luis Carrillo-Avila, Jose Antonio Chen, Qi Magtoto, Ronaldo Poonsuk, Korakrit Baum, David H. Piñeyro, Pablo Zimmerman, Jeffrey
dc.contributor.department Veterinary Diagnostic and Production Animal Medicine 2022-04-11T21:20:37Z 2022-04-11T21:20:37Z 2017-04-25
dc.description.abstract The development of porcine epidemic diarrhea virus (PEDV) antibody-based assays is important for detecting infected animals, confirming previous virus exposure, and monitoring sow herd immunity. However, the potential cross-reactivity among porcine coronaviruses is a major concern for the development of pathogen-specific assays. In this study, we used serum samples (n = 792) from pigs of precisely known infection status and a multiplex fluorescent microbead-based immunoassay and/or enzyme-linked immunoassay platform to characterize the antibody response to PEDV whole-virus (WV) particles and recombinant polypeptides derived from the four PEDV structural proteins, i.e., spike (S), nucleocapsid (N), membrane (M), and envelope (E). Antibody assay cutoff values were selected to provide 100% diagnostic specificity for each target. The earliest IgG antibody response, mainly directed against S1 polypeptides, was observed at days 7 to 10 postinfection. With the exception of nonreactive protein E, we observed similar antibody ontogenies and patterns of seroconversion for S1, N, M, and WV antigens. Recombinant S1 provided the best diagnostic sensitivity, regardless of the PEDV strain, with no cross-reactivity detected against transmissible gastroenteritis virus (TGEV), porcine respiratory coronavirus (PRCV), or porcine deltacoronavirus (PDCoV) pig antisera. The WV particles showed some cross-reactivity to TGEV Miller and TGEV Purdue antisera, while N protein presented some cross-reactivity to TGEV Miller. The M protein was highly cross-reactive to TGEV and PRCV antisera. Differences in the antibody responses to specific PEDV structural proteins have important implications in the development and performance of antibody assays for the diagnosis of PEDV enteric disease.
dc.description.comments This is a manuscript of an article published as Gimenez-Lirola, Luis Gabriel, Jianqiang Zhang, Jose Antonio Carrillo-Avila, Qi Chen, Ronaldo Magtoto, Korakrit Poonsuk, David H. Baum, Pablo Piñeyro, and Jeffrey Zimmerman. "Reactivity of anti-PEDV structural protein antibodies to porcine enteric coronaviruses: diagnostic implications." Journal of Clinical Microbiology (2017). DOI: 10.1128/JCM.02507-16. Copyright 2017 American Society for Microbiology. Posted with permission.
dc.language.iso en
dc.publisher American Society for Microbiology
dc.source.uri *
dc.subject.keywords PEDV
dc.subject.keywords recombinant structural proteins
dc.subject.keywords whole virus
dc.subject.keywords multiplex FMIA
dc.subject.keywords ELISA
dc.subject.keywords antibody response
dc.subject.keywords cross-reactivity
dc.title Reactivity of Porcine Epidemic Diarrhea Virus Structural Proteins to Antibodies against Porcine Enteric Coronaviruses: Diagnostic Implications
dc.type Article
dspace.entity.type Publication
relation.isAuthorOfPublication abbf44fd-a4a0-4859-8b5c-af2b853c9547
relation.isOrgUnitOfPublication 5ab07352-4171-4f53-bbd7-ac5d616f7aa8
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