Localization of individual calcium channels at the release face of a presynaptic nerve terminal

dc.contributor.author Haydon, Philip
dc.contributor.author Henderson, Eric
dc.contributor.author Henderson, Eric
dc.contributor.author Stanley, Elis
dc.contributor.department Zoology and Genetics
dc.date 2018-02-18T09:39:26.000
dc.date.accessioned 2020-07-07T05:16:48Z
dc.date.available 2020-07-07T05:16:48Z
dc.date.issued 1994-12-01
dc.description.abstract <p>Studies using biophysical techniques suggest a highly structured organization of <a href="http://topics.sciencedirect.com/topics/page/Calcium_channel">calcium channels</a> at the <a href="http://topics.sciencedirect.com/topics/page/Chemical_synapse">presynaptic</a> transmitter release face (<a href="http://www.sciencedirect.com/science/article/pii/0896627394904146#bib17" id="x-x-ancbbib17">Llinás et al., 1981</a>; <a href="http://www.sciencedirect.com/science/article/pii/0896627394904146#bib36" id="x-x-ancbbib36">Stanley, 1993</a>), but it has not as yet proved possible to localize identified channels at the required nanometer level of resolution. We have used atomic force microscopy on the calyx-type nerve terminal of the chick <a href="http://topics.sciencedirect.com/topics/page/Ciliary_ganglion">ciliary ganglion</a> to localize single calcium channels tagged via biotinylated ω-conotoxin GVIA to avidin-coated 30 nm gold particles. Calcium channels were in low (modal value approximately ⩽ 1 per μm<sup>2</sup>) and high (modal value55 per μm<sup>2</sup>) density areas and exhibited a prominent interchannel spacing of 40 nm, indicating an intermolecular linkage. Particles were observed in clusters and short linear or parallel linear arrays, groupings that may reflect <a href="http://topics.sciencedirect.com/topics/page/Calcium_channel">calcium channel</a> organization at the transmitter release site.</p>
dc.description.comments <p>This article is from <em>Neuron </em>13 (1994): 1275, doi: <a href="https://doi.org/10.1016/0896-6273(94)90414-6" id="x-ddDoi">10.1016/0896-6273(94)90414-6</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/zool_pubs/18/
dc.identifier.articleid 1022
dc.identifier.contextkey 10114859
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath zool_pubs/18
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92626
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/zool_pubs/18/1994_Henderson_LocalizationIndividual.pdf|||Fri Jan 14 21:34:16 UTC 2022
dc.source.uri 10.1016/0896-6273(94)90414-6
dc.subject.disciplines Cell Anatomy
dc.subject.disciplines Cell Biology
dc.subject.disciplines Developmental Biology
dc.subject.disciplines Genetics and Genomics
dc.title Localization of individual calcium channels at the release face of a presynaptic nerve terminal
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 68bc511a-afd6-4353-ae20-066de33682b9
relation.isOrgUnitOfPublication 4a2929da-5374-4338-b62f-f5fd9e156ef9
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