CRISPR/Cas9 Mediated Mutagenesis in Danio rerio

dc.contributor.author Bullard, Cassie
dc.contributor.department Genetics, Development and Cell Biology
dc.date 2018-02-17T00:11:44.000
dc.date.accessioned 2020-07-07T05:11:00Z
dc.date.available 2020-07-07T05:11:00Z
dc.date.issued 2015-04-14
dc.description.abstract <p>Zebrafish (Danio rerio) serve as a very useful model organism because they have a fast generation time, clear embryos and a well mapped genome. In this research project, the students in the Developmental Biology lab course and the students in the Freshmen Research Initiative have used these characteristics to conduct a screening of the zebrafish genome in order to identify genes that are required for development. The CRISPR/Cas9 system (a protein that creates double strand breaks at specific sites in the genome that are then repaired by the cellular machinery) was recently specialized for the use in zebrafish. However, there are usually mistakes made when repairing the break. By using this system we can create mutations at specific sites in the genome and even delete entire sections. We can then observe if the mutation has created any notable phenotypes in the developing embryo. That information can give us insight into what the genetic requirements are for development or how those mechanisms can go wrong in diseases such as cancer.</p>
dc.identifier archive/lib.dr.iastate.edu/undergradresearch_symposium/2015/presentations/16/
dc.identifier.articleid 1104
dc.identifier.contextkey 7558424
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath undergradresearch_symposium/2015/presentations/16
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/91677
dc.relation.ispartofseries Symposium on Undergraduate Research and Creative Expression
dc.source.bitstream archive/lib.dr.iastate.edu/undergradresearch_symposium/2015/presentations/16/2.C.1_20Bullard.pdf|||Fri Jan 14 20:51:49 UTC 2022
dc.subject.disciplines Developmental Biology
dc.subject.disciplines Genetics
dc.title CRISPR/Cas9 Mediated Mutagenesis in Danio rerio
dc.type event
dc.type.genre article
dspace.entity.type Publication
relation.isOrgUnitOfPublication 9e603b30-6443-4b8e-aff5-57de4a7e4cb2
relation.isSeriesOfPublication 6730f354-97b8-4408-bad3-7e5c3b2fca9d
thesis.degree.discipline Biology
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