Global transcriptional response of porcine mesenteric lymph nodes to Salmonella enterica serovar Typhimurium

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2007-07-01
Authors
Wang, Yanfang
Nettleton, Dan
Qu, Long
Uthe, Jolita
Bearson, Shawn
Kuhar, Daniel
Lunney, Joan
Couture, Oliver
Nettleton, Dan
Dekkers, Jack
Tuggle, Christopher
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Abstract

To elucidate the host transcriptional response to Salmonella enterica serovar Typhimurium, Affymetrix porcine GeneChip analysis of pig mesenteric lymph nodes was used to identify 848 genes showing differential expression across different times after inoculation or when compared to non-inoculated controls. Annotation analyses showed that a high proportion of these differentially expressed (DE) genes are involved in immune and inflammatory responses. T helper 1, innate/inflammatory, and antigen-processing pathways were induced at 24 h post-inoculation (hpi) and/or 48 hpi, while apoptosis and antigen presentation/dendritic cell function pathways were downregulated at 8 hpi. Cluster analyses revealed that most DE genes annotated as NFκB targets were grouped into a specific induced subcluster, while many translation-related DE genes were found in a repressed subcluster. Quantitative polymerase chain reaction analyses confirmed the Affymetrix results, revealing transcriptional induction of NFκB target genes at 24 hpi and suppression of the NFκB pathway from 24 to 48 hpi. We propose that such NFκB suppression in antigen-presenting cells may be the mechanism by which S. Typhimurium eludes a strong inflammatory response to establish a carrier status in pigs.

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This article is published as Wang, Yanfang, Long Qu, Jolita J. Uthe, Shawn MD Bearson, Daniel Kuhar, Joan K. Lunney, Oliver P. Couture, Dan Nettleton, Jack CM Dekkers, and Christopher K. Tuggle. "Global transcriptional response of porcine mesenteric lymph nodes to Salmonella enterica serovar Typhimurium." Genomics 90, no. 1 (2007): 72-84. doi: 10.1016/j.ygeno.2007.03.018.

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