A single-stranded DNA binding protein that specifically recognizes cis-acting sequences in the replication origin and transcriptional promoter region of Tetrahymena rDNA
dc.contributor.author | Hou, Zhen | |
dc.contributor.author | Umthun, Angela | |
dc.contributor.author | Dobbs, Drena | |
dc.contributor.department | Zoology and Genetics | |
dc.contributor.department | Molecular, Cellular and Developmental Biology | |
dc.contributor.department | Genetics | |
dc.date | 2018-02-18T14:19:32.000 | |
dc.date.accessioned | 2020-07-07T05:16:59Z | |
dc.date.available | 2020-07-07T05:16:59Z | |
dc.date.copyright | Sun Jan 01 00:00:00 UTC 1995 | |
dc.date.issued | 1995 | |
dc.description.abstract | <p>Type I repeat sequences are evolutionarily conserved sequence elements found in the replication origin and transcriptional promoter region of the rRNA genes (rDNA) in Tetrahymena thermophila. An abundant single-stranded DNA binding protein, ssA-TIBF, specifically interacts with the A-rich strand of the Type I repeat sequence. Quantitative binding competition experiments performed with purified ssATIBF demonstrate that the binding site for ssA-TIBF includes sequences both within the conserved 33 nt element and in a 3' flanking region: addition of the 3' flanking sequence to the Type I repeat oligonucleotide increases the binding affinity of ssA-TIBF by nearly 100-fold (apparent K& = 3.0 x 10~10 M). A mutation in the ssA-TIBF binding site previously shown to be the determinant of an rDNA replication defect in vivo results in a 25-fold decrease in ssA-TIBF binding affinity in vitro. ssA-TIBF also binds with high affinity to a copy of the Type I repeat sequence within the essential promoter region defined by in vitro transcription assays. The affinity of ssA-TIBF for the promoter repeat, which differs from other copies of the repeat at 8 out of 33 positions, is at least equal to its affinity for the Type I repeat sequences in the origin region. The biochemical properties of ssA-TIBF in vitro suggest that it could play a role in both replication and transcription of Tetrahymena rDNA in vivo.</p> | |
dc.description.comments | <p>Reprinted (adapted) with permission from A single-stranded DNA binding protein that specifically recognizes cis-acting sequences in the replication origin and transcriptional promoter region of Tetrahymena rDNA, Zhen Hou, Angela R. Umthun, and Drena L. Dobbs, Biochemistry 1995 34 (14), 4583-4592, DOI: <a href="http://dx.doi.org/10.1021/bi00014a011" target="_blank">10.1021/bi00014a011</a>. Copyright 1995 American Chemical Society.</p> | |
dc.format.mimetype | application/pdf | |
dc.identifier | archive/lib.dr.iastate.edu/zool_pubs/47/ | |
dc.identifier.articleid | 1045 | |
dc.identifier.contextkey | 10357080 | |
dc.identifier.s3bucket | isulib-bepress-aws-west | |
dc.identifier.submissionpath | zool_pubs/47 | |
dc.identifier.uri | https://dr.lib.iastate.edu/handle/20.500.12876/92658 | |
dc.language.iso | en | |
dc.source.bitstream | archive/lib.dr.iastate.edu/zool_pubs/47/0-Dobbs_Permission_for_ACS_1995_Single.pdf|||Sat Jan 15 00:24:57 UTC 2022 | |
dc.source.bitstream | archive/lib.dr.iastate.edu/zool_pubs/47/1995_Dobbs_SingleStranded.pdf|||Sat Jan 15 00:24:58 UTC 2022 | |
dc.source.uri | 10.1021/bi00014a011 | |
dc.subject.disciplines | Cell and Developmental Biology | |
dc.subject.disciplines | Genetics | |
dc.subject.disciplines | Genomics | |
dc.subject.disciplines | Molecular Genetics | |
dc.title | A single-stranded DNA binding protein that specifically recognizes cis-acting sequences in the replication origin and transcriptional promoter region of Tetrahymena rDNA | |
dc.type | article | |
dc.type.genre | article | |
dspace.entity.type | Publication | |
relation.isOrgUnitOfPublication | 4a2929da-5374-4338-b62f-f5fd9e156ef9 |