Interaction between Rag genes results in a unique synergistic transcriptional response that enhances soybean resistance to soybean aphids

dc.contributor.author Natukunda, Martha
dc.contributor.author Hohenstein, Jessica
dc.contributor.author McCabe, Chantal
dc.contributor.author Graham, Michelle
dc.contributor.author Qi, Yunhui
dc.contributor.author Singh, Asheesh
dc.contributor.author MacIntosh, Gustavo
dc.contributor.department Biochemistry, Biophysics and Molecular Biology
dc.contributor.department Agronomy
dc.contributor.department Statistics
dc.date.accessioned 2022-01-31T14:52:35Z
dc.date.available 2022-01-31T14:52:35Z
dc.date.issued 2021-12-11
dc.description.abstract Background: Pyramiding different resistance genes into one plant genotype confers enhanced resistance at the phenotypic level, but the molecular mechanisms underlying this effect are not well-understood. In soybean, aphid resistance is conferred by Rag genes. We compared the transcriptional response of four soybean genotypes to aphid feeding to assess how the combination of Rag genes enhanced the soybean resistance to aphid infestation. Results: A strong synergistic interaction between Rag1 and Rag2, defined as genes differentially expressed only in the pyramid genotype, was identified. This synergistic effect in the Rag1/2 phenotype was very evident early (6 h after infestation) and involved unique biological processes. However, the response of susceptible and resistant genotypes had a large overlap 12 h after aphid infestation. Transcription factor (TF) analyses identified a network of interacting TF that potentially integrates signaling from Rag1 and Rag2 to produce the unique Rag1/2 response. Pyramiding resulted in rapid induction of phytochemicals production and deposition of lignin to strengthen the secondary cell wall, while repressing photosynthesis. We also identified Glyma.07G063700 as a novel, strong candidate for the Rag1 gene. Conclusions: The synergistic interaction between Rag1 and Rag2 in the Rag1/2 genotype can explain its enhanced resistance phenotype. Understanding molecular mechanisms that support enhanced resistance in pyramid genotypes could facilitate more directed approaches for crop improvement.
dc.description.comments This article is published as Natukunda, M.I., Hohenstein, J.D., McCabe, C.E. et al. Interaction between Rag genes results in a unique synergistic transcriptional response that enhances soybean resistance to soybean aphids. BMC Genomics 22, 887 (2021). doi:10.1186/s12864-021-08147-3. Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/qzXB5gYv
dc.language.iso en
dc.source.uri https://doi.org/10.1186/s12864-021-08147-3 *
dc.subject Gene pyramiding
dc.subject Soybean
dc.subject Soybean aphids
dc.subject Aphid resistance
dc.subject Rag genes
dc.subject RNA sequencing
dc.subject Synergistic effect
dc.subject.disciplines DegreeDisciplines::Life Sciences::Biochemistry, Biophysics, and Structural Biology
dc.subject.disciplines DegreeDisciplines::Life Sciences::Agriculture
dc.subject.disciplines DegreeDisciplines::Life Sciences::Plant Sciences::Agronomy and Crop Sciences
dc.title Interaction between Rag genes results in a unique synergistic transcriptional response that enhances soybean resistance to soybean aphids
dc.type Article
dspace.entity.type Publication
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