Updating Quality Scores During HMM-Based Correction of Illumina Next Generation Sequencing Data

Thumbnail Image
Date
2021-01-01
Authors
Zhang, Haijuan
Major Professor
Karin Dorman
Advisor
Committee Member
Journal Title
Journal ISSN
Volume Title
Publisher
Altmetrics
Abstract

There are many error correction tools to remove the base calling errors made by Illumina technology, but most do not update the quality scores, even after correcting the errors. The quality score is an important metric quantifying the trustworthiness of the corresponding base call that is used by many downstream sequence analysis tools. This research proposes a method to update quality scores of corrected errors when using PREMIER, a fully-probabilistic error correction method for Illumina sequencing data. I then test the quality of the updates to see if the updated quality scores better reflect the actual probability of error in an Illumina dataset.

Series Number
Journal Issue
Is Version Of
Versions
Series
Academic or Administrative Unit
Type
creative component
Comments
Rights Statement
Copyright
Fri Jan 01 00:00:00 UTC 2021
Funding
Subject Categories
Supplemental Resources
Source