Polymorphisms in O-methyltransferase genes are associated with stover cell wall digestibility in European maize (Zea maysL.)

dc.contributor.author Brenner, Everton
dc.contributor.author Zein, Imad
dc.contributor.author Lubberstedt, Thomas
dc.contributor.author Chen, Yongsheng
dc.contributor.author Andersen, Jeppe
dc.contributor.author Wenzel, Gerhard
dc.contributor.author Ouzunova, Milena
dc.contributor.author Eder, Joachim
dc.contributor.author Darnhofer, Birte
dc.contributor.author Frei, Uschi
dc.contributor.author Barrière, Yves
dc.contributor.author Lubberstedt, Thomas
dc.contributor.department Agronomy
dc.date 2018-02-19T00:20:39.000
dc.date.accessioned 2020-06-29T23:03:56Z
dc.date.available 2020-06-29T23:03:56Z
dc.date.copyright Fri Jan 01 00:00:00 UTC 2010
dc.date.issued 2010-02-12
dc.description.abstract <p><h3>Background</h3></p> <p>OMT (O-methyltransferase) genes are involved in lignin biosynthesis, which relates to stover cell wall digestibility. Reduced lignin content is an important determinant of both forage quality and ethanol conversion efficiency of maize stover. <h3>Results</h3></p> <p>Variation in genomic sequences coding for <em>COMT, CCoAOMT1</em>, and <em>CCoAOMT2</em> was analyzed in relation to stover cell wall digestibility for a panel of 40 European forage maize inbred lines, and re-analyzed for a panel of 34 lines from a published French study. Different methodologies for association analysis were performed and compared. Across association methodologies, a total number of 25, 12, 1, 6 <em>COMT</em> polymorphic sites were significantly associated with DNDF, OMD, NDF, and WSC, respectively. Association analysis for <em>CCoAOMT1</em> and <em>CCoAOMT2</em> identified substantially fewer polymorphic sites (3 and 2, respectively) associated with the investigated traits. Our re-analysis on the 34 lines from a published French dataset identified 14 polymorphic sites significantly associated with cell wall digestibility, two of them were consistent with our study. Promising polymorphisms putatively causally associated with variability of cell wall digestibility were inferred from the total number of significantly associated SNPs/Indels. <h3>Conclusions</h3></p> <p>Several polymorphic sites for three O-methyltransferase loci were associated with stover cell wall digestibility. All three tested genes seem to be involved in controlling DNDF, in particular <em>COMT</em>. Thus, considerable variation among <em>Bm3</em> wildtype alleles can be exploited for improving cell-wall digestibility. Target sites for functional markers were identified enabling development of efficient marker-based selection strategies.</p>
dc.description.comments <p>This article is published as Brenner, Everton A., Imad Zein, Yongsheng Chen, Jeppe R. Andersen, Gerhard Wenzel, Milena Ouzunova, Joachim Eder et al. "Polymorphisms in O-methyltransferase genes are associated with stover cell wall digestibility in European maize (Zea mays L.)." BMC plant biology 10, no. 1 (2010): 27. doi: <a href="https://doi.org/10.1186/1471-2229-10-27" target="_blank">10.1186/1471-2229-10-27</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/agron_pubs/279/
dc.identifier.articleid 1270
dc.identifier.contextkey 10950925
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath agron_pubs/279
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/4619
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/agron_pubs/279/2010_Lubberstedt_PolymorphismsO.pdf|||Fri Jan 14 23:08:22 UTC 2022
dc.source.uri 10.1186/1471-2229-10-27
dc.subject.disciplines Agronomy and Crop Sciences
dc.title Polymorphisms in O-methyltransferase genes are associated with stover cell wall digestibility in European maize (Zea maysL.)
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 4e4330cd-db15-4ac5-8924-41119139cf32
relation.isOrgUnitOfPublication fdd5c06c-bdbe-469c-a38e-51e664fece7a
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