Quantification of major bovine milk proteins
Date
1997
Authors
Bode, Gerd
Major Professor
Advisor
Lindberg, Gary L.
Freeman, Albert E.
Committee Member
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Abstract
A method is described for qualitative and quantitative determinations of the major bovine milk proteins and some of their phenotypic variants by reversed-phase high-performance liquid chromatography. The six major proteins in bovine milk can be resolved in a single run and quantified in less than 1 h. The quantitative response was linear for each major milk protein. The relative standard deviation of the quantitative response was for each major milk protein within days below 5.1% and between days 7.1%. When the method was used to determine the milk protein composition of 234 bovine milk samples the results were similar to published values for milk protein composition.
Effects of sample preparation on behavior of milk proteins during reversed-phase highperformance liquid chromatography were examined. Peak areas of individual milk proteins were influenced by types of reducing and chaotropic agents and time interval between sample preparation and injection. Cysteine-containing and globular proteins were influenced most strongly. Equilibrium of different structural forms of proteins could explain their differential responses to preparation conditions. Peak heights responded more sensitively to sample preparation conditions than did peak area.
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thesis