Cytotoxicity assessment of Aflatoxin B1 after high voltage atmospheric cold plasma treatment

Date
2021-04-30
Authors
Nishimwe, Kizito
Agbemafle, Isaac
Reddy, Manju
Maier, Dirk
Keener, Kevin
Maier, Dirk
Major Professor
Advisor
Committee Member
Journal Title
Journal ISSN
Volume Title
Publisher
Altmetrics
Authors
Maier, Dirk
Person
Research Projects
Organizational Units
Journal Issue
Series
Department
Food Science and Human NutritionAgricultural and Biosystems Engineering
Abstract

Aflatoxin B1 (AFB1) is a secondary metabolite produced by Aspergillus flavus and A. parasiticus, and is a known carcinogen in humans and animals. High voltage atmospheric cold plasma (HVACP) technology has already shown promise to decontaminate AFB1 in food and feed. This study aimed to investigate the cytotoxicity of AFB1 after HVACP treatment. AFB1 (100 μM) was treated at 85 kV with HVACP for 0, 2, 5, 10, and 20 min. HepG2 cells were exposed to HVACP-treated AFB1 for 72 h and assessed for cell viability, caspase-3 activity, DNA fragmentation, and protein carbonyls for each treatment time. Cell viability, caspase-3 activity, DNA fragmentation levels, and protein carbonyls contents of HepG2 cells exposed to HVACP-treated AFB1 after 20 min was not significantly different compared to non-exposed HepG2 cells (P > 0.05). However, their contents were significantly higher in non-exposed cells compared to the other HVACP treatment times (P < 0.01). Twenty minutes of HVACP treatment for AFB1 significantly reduced AFB1 cytotoxicity and oxidative damage and showed potential as a safe aflatoxin decontamination technology.

Comments

This is a manuscript of an article published as Nishimwe, Kizito, Isaac Agbemafle, Manju B. Reddy, Kevin Keener, and Dirk E. Maier. "Cytotoxicity Assessment of Aflatoxin B1 after High Voltage Atmospheric Cold Plasma Treatment." 194 Toxicon (2021): 17. DOI: 10.1016/j.toxicon.2021.02.008. Posted with permission.

Description
Keywords
Citation
DOI
Collections