Spatial Structuring of Cellulase Gene Abundance and Activity in Soil

dc.contributor.author Choi, Jinlyung
dc.contributor.author Bach, Elizabeth
dc.contributor.author Lee, Jaejin
dc.contributor.author Flater, Jared
dc.contributor.author Dooley, Shane
dc.contributor.author Howe, Adina
dc.contributor.author Hofmockel, Kirsten
dc.contributor.department Ecology, Evolution and Organismal Biology
dc.contributor.department Agricultural and Biosystems Engineering
dc.date 2018-10-11T19:57:26.000
dc.date.accessioned 2020-06-29T22:43:59Z
dc.date.available 2020-06-29T22:43:59Z
dc.date.copyright Mon Jan 01 00:00:00 UTC 2018
dc.date.issued 2018-10-02
dc.description.abstract <p>Microbial mechanisms controlling cellulose degradation in soil habitats remains a critical knowledge gap in understanding and modeling terrestrial carbon-cycling. We investigated land management and soil micro-habitat influences on soil bacterial communities and distribution of cellulose-degrading enzyme genes in three bioenergy cropping systems (corn, prairie, and fertilized prairie). Within the soil, aggregates have been examined as potential micro- habitats with specific characteristics influencing resource partitioning and regulation, thus we also investigated genes associated with cellulose degradation within soil aggregate fractions from the fertilized prairie system. Soil bacterial communities and carbon-cycling gene presence varied across land management and soil microhabitats. Examination of genes specifically involved in cellulose-degradation pathways showed high levels of redundancy across the bioenergy cropping systems, but medium macroaggregates (1,000–2,000 μm) supported greater cellulose-degrading enzyme gene abundance than other aggregate fractions and whole soil. In medium aggregates, the enriched cellulose-degrading genes were most similar to genes previously observed in Actinobacteria. These findings represent gentic potential only, and our previous work on the same samples found elevated cellulase exo-enzyme activity in microaggregates. These contrasting results emphasize the importance of measuring community, functional genes, and metabolic potentials in a coordinated manner. Together, these data indicate that location within the soil matrix matters. Overall, our results indicate that soil aggregate environments are hot-spots that select for organisms with functional attributes like cellulose degradation, and future work should further explore micro-environmental factors that affect realized C-cycling processes.</p>
dc.description.comments <p>This article is published as Choi, Jinlyung, Elizabeth Bach, Jaejin Lee, Jared Flater, Shane Dooley, Adina Howe, and Kirsten Hofmockel. "Spatial structuring of cellulase gene abundance and activity in soil." <em>Frontiers in Environmental Science</em> 6 (2018): 107. DOI: <a href="https://dx.doi.org/10.3389/fenvs.2018.00107" target="_blank">10.3389/fenvs.2018.00107</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/abe_eng_pubs/956/
dc.identifier.articleid 2239
dc.identifier.contextkey 13016546
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath abe_eng_pubs/956
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/1775
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/abe_eng_pubs/956/2018_Howe_SpatialStructuring.pdf|||Sat Jan 15 02:34:40 UTC 2022
dc.source.uri 10.3389/fenvs.2018.00107
dc.subject.disciplines Agriculture
dc.subject.disciplines Bioresource and Agricultural Engineering
dc.subject.disciplines Environmental Sciences
dc.subject.disciplines Genetics
dc.subject.disciplines Soil Science
dc.subject.keywords microbiome
dc.subject.keywords carbon cycling
dc.subject.keywords metagenomes
dc.subject.keywords aggregates
dc.subject.keywords prairie
dc.subject.keywords bioenergy
dc.title Spatial Structuring of Cellulase Gene Abundance and Activity in Soil
dc.type article
dc.type.genre article
dspace.entity.type Publication
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relation.isOrgUnitOfPublication 8eb24241-0d92-4baf-ae75-08f716d30801
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