A Self-Referencing Detection of Microorganisms Using Surface Enhanced Raman Scattering Nanoprobes in a Test-in-a-Tube Platform

dc.contributor.author Xiao, Nan
dc.contributor.author Yu, Chenxu
dc.contributor.author Wang, Chao
dc.contributor.author Yu, Chenxu
dc.contributor.department Agricultural and Biosystems Engineering
dc.date 2018-02-13T16:47:34.000
dc.date.accessioned 2020-06-29T22:39:53Z
dc.date.available 2020-06-29T22:39:53Z
dc.date.copyright Tue Jan 01 00:00:00 UTC 2013
dc.date.embargo 2013-10-31
dc.date.issued 2013-09-13
dc.description.abstract <p>Anisotropic nanoparticles (<em>i.e</em>., silver nanocubes) were functionalized with target-specific antibodies and Raman active tags to serve as nanoprobes for the rapid detection of bacteria in a test-in-a-tube platform. A self-referencing scheme was developed and implemented in which surface enhanced Raman spectroscopic (SERS) signatures of the targets were observed superimposed with the SERS signals of the Raman tags. The assessment through the dual signals (superimposed target and tag Raman signatures) supported a specific recognition of the targets in a single step with no washing/separation needed to a sensitivity of 10<sup>2</sup> CFU/mL, even in the presence of non-target bacteria at a 10 times higher concentration. The self-referencing protocol implemented with a portable Raman spectrometer potentially can become an easy-to-use, field-deployable spectroscopic sensor for onsite detection of pathogenic microorganisms.</p>
dc.description.comments <p>This article is from <em>Biosensors</em> 3 (2013): 312–326, doi:<a href="http://dx.doi.org/10.3390/bios3030312" target="_blank">10.3390/bios3030312</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/abe_eng_pubs/444/
dc.identifier.articleid 1723
dc.identifier.contextkey 4782293
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath abe_eng_pubs/444
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/1211
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/abe_eng_pubs/444/2013_XiaoN_ASelfReferencingDetection.pdf|||Sat Jan 15 00:18:42 UTC 2022
dc.source.uri 10.3390/bios3030312
dc.subject.disciplines Agriculture
dc.subject.disciplines Bioresource and Agricultural Engineering
dc.subject.keywords surface enhanced Raman scattering
dc.subject.keywords self-referencing
dc.subject.keywords microorganism
dc.subject.keywords nanoparticles
dc.title A Self-Referencing Detection of Microorganisms Using Surface Enhanced Raman Scattering Nanoprobes in a Test-in-a-Tube Platform
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 837654ad-03e3-4004-b198-68d7756bcd27
relation.isOrgUnitOfPublication 8eb24241-0d92-4baf-ae75-08f716d30801
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