Morphological Transformations in the Magnetite Biomineralizing Protein Mms6 in Iron Solutions: A Small-Angle X-ray Scattering Study Mallapragada, Surya Liu, Xunpei Feng, Shuren Wang, Wenjie Schmidt-Rohr, Klaus Akinc, Mufit Nilsen-Hamilton, Marit Vaknin, David Nilsen-Hamilton, Marit
dc.contributor.department Biochemistry, Biophysics and Molecular Biology
dc.contributor.department Ames Laboratory
dc.contributor.department Physics and Astronomy
dc.contributor.department Materials Science and Engineering
dc.contributor.department Chemical and Biological Engineering
dc.contributor.department Chemistry 2018-02-17T10:52:46.000 2020-06-29T23:47:06Z 2020-06-29T23:47:06Z Thu Jan 01 00:00:00 UTC 2015 2015-01-01
dc.description.abstract <p>Magnetotactic bacteria that produce magnetic nanocrystals of uniform size and well-defined morphologies have inspired the use of biomineralization protein Mms6 to promote formation of uniform magnetic nanocrystals <em>in vitro</em>. Small angle X-ray scattering (SAXS) studies in physiological solutions reveal that Mms6 forms compact globular three-dimensional (3D) micelles (approximately 10 nm in diameter) that are, to a large extent, independent of concentration. In the presence of iron ions in the solutions, the general micellar morphology is preserved, however, with associations among micelles that are induced by iron ions. Compared with Mms6, the m2Mms6 mutant (with the sequence of hydroxyl/carboxyl containing residues in the C-terminal domain shuffled) exhibits subtle morphological changes in the presence of iron ions in solutions. The analysis of the SAXS data is consistent with a hierarchical core–corona micellar structure similar to that found in amphiphilic polymers. The addition of ferric and ferrous iron ions to the protein solution induces morphological changes in the micellar structure by transforming the 3D micelles into objects of reduced dimensionality of 2, with fractal-like characteristics (including Gaussian-chain-like) or, alternatively, platelet-like structures.</p>
dc.description.comments <p>Reprinted (adapted) with permission from Langmuir, 2015, 31 (9), pp 2818–2825<strong>, <a href="" target="_blank">DOI: </a></strong><a href="" target="_blank">10.1021/la5044377</a>. Copyright (2015) American Chemical Society.</p>
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dc.identifier archive/
dc.identifier.articleid 1038
dc.identifier.contextkey 8029835
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath bbmb_ag_pubs/35
dc.language.iso en
dc.source.bitstream archive/|||Fri Jan 14 23:43:45 UTC 2022
dc.source.bitstream archive/|||Fri Jan 14 23:43:47 UTC 2022
dc.source.uri 10.1021/la5044377
dc.subject.disciplines Biochemistry, Biophysics, and Structural Biology
dc.subject.disciplines Biological and Chemical Physics
dc.subject.disciplines Materials Chemistry
dc.subject.disciplines Organic Chemistry
dc.subject.disciplines Other Chemistry
dc.title Morphological Transformations in the Magnetite Biomineralizing Protein Mms6 in Iron Solutions: A Small-Angle X-ray Scattering Study
dc.type article
dc.type.genre article
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