CmeR Functions as a Transcriptional Repressor for the Multidrug Efflux Pump CmeABC in Campylobacter jejuni

dc.contributor.author Lin, Jun
dc.contributor.author Zhang, Qijing
dc.contributor.author Akiba, Masato
dc.contributor.author Sahin, Orhan
dc.contributor.author Zhang, Qijing
dc.contributor.department Veterinary Microbiology and Preventive Medicine
dc.date 2018-02-18T23:27:50.000
dc.date.accessioned 2020-07-07T05:14:47Z
dc.date.available 2020-07-07T05:14:47Z
dc.date.copyright Sat Jan 01 00:00:00 UTC 2005
dc.date.issued 2005-03-01
dc.description.abstract <p>CmeABC, a resistance-nodulation-division (RND) type of efflux pump, contributes to <em>Campylobacter</em> resistance to a broad spectrum of antimicrobial agents and is also essential for <em>Campylobacter</em> colonization of the animal intestinal tract by mediation of bile resistance. As one of the main systems for <em>Campylobacter</em> adaptation to different environments, CmeABC is likely subject to control by regulatory elements. We describe the identification of a transcriptional repressor for CmeABC. Insertional mutagenesis of <em>cmeR</em>, an open reading frame immediately upstream of the <em>cmeABC</em> operon, resulted in overexpression of <em>cmeABC</em>, as determined by transcriptional fusion (P<sub><em>cmeABC-lacZ</em></sub>) and immunoblotting with CmeABC-specific antibodies. Overexpression of the efflux pump was correlated with a moderate increase in the level of resistance of the <em>cmeR</em> mutant to several antimicrobials. In vitro, recombinant CmeR bound specifically to the promoter region of <em>cmeABC</em>, precisely, to the inverted repeat sequences in the <em>cmeABC</em> promoter. A single nucleotide deletion between the two half sites of the inverted repeat reduced the level of CmeR binding to the promoter sequence and resulted in overexpression of <em>cmeABC</em>. Together, these findings indicate that <em>cmeR</em> encodes a transcriptional repressor that directly interacts with the <em>cmeABC</em> promoter and modulates the expression of <em>cmeABC</em>. Mutation either in CmeR or in the inverted repeat impedes the repression and leads to enhanced production of the MDR efflux pump.</p>
dc.description.comments <p>This article is published as Lin, Jun, Masato Akiba, Orhan Sahin, and Qijing Zhang. "CmeR functions as a transcriptional repressor for the multidrug efflux pump CmeABC in Campylobacter jejuni." Antimicrobial agents and chemotherapy 49, no. 3 (2005): 1067-1075. doi: <a href="http://dx.doi.org/10.1128/AAC.49.3.1067-1075.2005%20" target="_blank" title=" CmeR Functions as a Transcriptional Repressor for the Multidrug Efflux Pump CmeABC in Campylobacter jejuni">10.1128/AAC.49.3.1067-1075.2005</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/vmpm_pubs/172/
dc.identifier.articleid 1172
dc.identifier.contextkey 10837478
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vmpm_pubs/172
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92279
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vmpm_pubs/172/2005_Zhang_FunctionsTranscriptional.pdf|||Fri Jan 14 21:18:29 UTC 2022
dc.source.uri 10.1128/AAC.49.3.1067-1075.2005
dc.subject.disciplines Genetics and Genomics
dc.subject.disciplines Molecular Genetics
dc.subject.disciplines Veterinary Microbiology and Immunobiology
dc.subject.disciplines Veterinary Preventive Medicine, Epidemiology, and Public Health
dc.title CmeR Functions as a Transcriptional Repressor for the Multidrug Efflux Pump CmeABC in Campylobacter jejuni
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 1c6a5dfc-c604-457f-85be-122910db782e
relation.isOrgUnitOfPublication 16f8e472-b1cd-4d8f-b016-09e96dbc4d83
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