CmeR Functions as a Transcriptional Repressor for the Multidrug Efflux Pump CmeABC in Campylobacter jejuni Lin, Jun Zhang, Qijing Akiba, Masato Sahin, Orhan Zhang, Qijing
dc.contributor.department Veterinary Microbiology and Preventive Medicine 2018-02-18T23:27:50.000 2020-07-07T05:14:47Z 2020-07-07T05:14:47Z Sat Jan 01 00:00:00 UTC 2005 2005-03-01
dc.description.abstract <p>CmeABC, a resistance-nodulation-division (RND) type of efflux pump, contributes to <em>Campylobacter</em> resistance to a broad spectrum of antimicrobial agents and is also essential for <em>Campylobacter</em> colonization of the animal intestinal tract by mediation of bile resistance. As one of the main systems for <em>Campylobacter</em> adaptation to different environments, CmeABC is likely subject to control by regulatory elements. We describe the identification of a transcriptional repressor for CmeABC. Insertional mutagenesis of <em>cmeR</em>, an open reading frame immediately upstream of the <em>cmeABC</em> operon, resulted in overexpression of <em>cmeABC</em>, as determined by transcriptional fusion (P<sub><em>cmeABC-lacZ</em></sub>) and immunoblotting with CmeABC-specific antibodies. Overexpression of the efflux pump was correlated with a moderate increase in the level of resistance of the <em>cmeR</em> mutant to several antimicrobials. In vitro, recombinant CmeR bound specifically to the promoter region of <em>cmeABC</em>, precisely, to the inverted repeat sequences in the <em>cmeABC</em> promoter. A single nucleotide deletion between the two half sites of the inverted repeat reduced the level of CmeR binding to the promoter sequence and resulted in overexpression of <em>cmeABC</em>. Together, these findings indicate that <em>cmeR</em> encodes a transcriptional repressor that directly interacts with the <em>cmeABC</em> promoter and modulates the expression of <em>cmeABC</em>. Mutation either in CmeR or in the inverted repeat impedes the repression and leads to enhanced production of the MDR efflux pump.</p>
dc.description.comments <p>This article is published as Lin, Jun, Masato Akiba, Orhan Sahin, and Qijing Zhang. "CmeR functions as a transcriptional repressor for the multidrug efflux pump CmeABC in Campylobacter jejuni." Antimicrobial agents and chemotherapy 49, no. 3 (2005): 1067-1075. doi: <a href="" target="_blank" title=" CmeR Functions as a Transcriptional Repressor for the Multidrug Efflux Pump CmeABC in Campylobacter jejuni">10.1128/AAC.49.3.1067-1075.2005</a>. Posted with permission.</p>
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dc.identifier archive/
dc.identifier.articleid 1172
dc.identifier.contextkey 10837478
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vmpm_pubs/172
dc.language.iso en
dc.source.bitstream archive/|||Fri Jan 14 21:18:29 UTC 2022
dc.source.uri 10.1128/AAC.49.3.1067-1075.2005
dc.subject.disciplines Genetics and Genomics
dc.subject.disciplines Molecular Genetics
dc.subject.disciplines Veterinary Microbiology and Immunobiology
dc.subject.disciplines Veterinary Preventive Medicine, Epidemiology, and Public Health
dc.title CmeR Functions as a Transcriptional Repressor for the Multidrug Efflux Pump CmeABC in Campylobacter jejuni
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 1c6a5dfc-c604-457f-85be-122910db782e
relation.isOrgUnitOfPublication 16f8e472-b1cd-4d8f-b016-09e96dbc4d83
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