Evaluation of the Antimicrobial Activity of Natural Animal Proteins/Peptides In Vitro

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dc.contributor.author Greiner, L.
dc.contributor.author Stahly, T.
dc.contributor.author Stanton, T.
dc.date 2018-02-12T23:57:33.000
dc.date.accessioned 2020-07-02T07:01:01Z
dc.date.available 2020-07-02T07:01:01Z
dc.date.copyright Mon Jan 01 00:00:00 UTC 2001
dc.date.embargo 2012-08-13
dc.date.issued 2001-01-01
dc.description.abstract <p>The objectives of this research were to validate the sensitivity and precision of an <em>in vitro</em> assay for evaluating the efficacy of antimicrobials, to evaluate the ability of natural animal proteins/peptides to kill <em>in vitro</em> antibiotic-resistant, as well as, -susceptible bacteria, and to determine the effects of key components of animal digesta (e.g., pH, mineral content, and proteolytic digestive enzymes) on the estimated antimicrobial activity of these proteins/peptides.</p> <p>The minimum inhibitory concentrations (MIC) for polymyxin B (control antibiotic) were determined to be .76, .76, and .90 µg/mL for <em>Escherichia coli</em>, <em>Escherichia coli</em> (nalidixic acid-resistant), and <em>Staphylococcus aureus</em>, respectively. The intra- and inter-assay variation for MIC determination was .18 and .2%, respectively.</p> <p>The natural animal proteins and peptides (lactoferrin, lactoferricin B, hen egg lysozyme, and alpha-lactalbumin LDT2) were determined in<em> in vitro</em> (acetic acid medium) to kill selected bacteria. Each of the tested proteins/peptides was active against an antibiotic-resistant (nalidixic acid) strain of <em>E. coli</em>; however, the required concentrations for antimicrobial activity were 10 to 15 times higher than that of the nonantibiotic-resistant strain. The antimicrobial activity of each protein/peptide in animal digesta fluid was 130 to 300% greater than that in the acetic acid media. Overall, the intra- and inter-assay variation values for the tested proteins/peptides was 3 and 3.4%, respectively.</p> <p>The antimicrobial activity of two of the three proteins/peptides was not affected by the presence of cationic minerals. The change in pH (digesta fluid and acetic acid media) from 7 to 2 resulted in a loss of antimicrobial activity of 33% for all proteins/peptides. Therefore, the increase in antimicrobial activity associated with the digesta fluid is not related to change in H or the mineral concentration of the digesta. Based on these data, natural proteins/peptides represent potential antibiotic substitutes.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/swinereports_2000/1/
dc.identifier.articleid 1014
dc.identifier.contextkey 3209911
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath swinereports_2000/1
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/91229
dc.language.iso en
dc.relation.ispartofseries Animal Science Research Reports
dc.relation.ispartofseries ASL R658
dc.source.bitstream archive/lib.dr.iastate.edu/swinereports_2000/1/asl_658.pdf|||Fri Jan 14 17:27:50 UTC 2022
dc.subject.disciplines Agriculture
dc.subject.disciplines Animal Sciences
dc.subject.keywords ASL R658
dc.title Evaluation of the Antimicrobial Activity of Natural Animal Proteins/Peptides In Vitro
dc.type article
dc.type.genre nutrition
dspace.entity.type Publication
relation.isSeriesOfPublication 7f3839b7-b833-4418-a6fa-adda2b23950a
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