Characterizing Avian Escherichia coli Isolates with Multiplex Polymerase Chain Reaction

dc.contributor.author Skyberg, Jerod
dc.contributor.author Nolan, Lisa
dc.contributor.author Horne, Shelley
dc.contributor.author Giddings, Catherine
dc.contributor.author Wooley, Richard
dc.contributor.author Gibbs, Penelope
dc.contributor.author Nolan, Lisa
dc.contributor.department Veterinary Microbiology and Preventive Medicine
dc.date 2018-02-13T10:47:13.000
dc.date.accessioned 2020-07-07T05:15:14Z
dc.date.available 2020-07-07T05:15:14Z
dc.date.copyright Wed Jan 01 00:00:00 UTC 2003
dc.date.embargo 2013-05-08
dc.date.issued 2003-10-01
dc.description.abstract <p>Colibacillosis caused by <em>Escherichia coli</em> infections account for significant morbidity and mortality in the poultry industry. Yet, despite the importance of colibacillosis, much about the virulence mechanisms employed by avian <em>E. coli</em> remains unknown. In recent years several genes have been linked to avian <em>E. coli</em> virulence, many of which reside on a large transmissible plasmid. In the present study, a multiplex polymerase chain reaction (PCR) protocol to detect the presence of four of these genes is described. Such a protocol may supplement current diagnostic schemes and provide a rapid means of characterizing the <em>E. coli</em> causing disease in poultry. The targets of this procedure included <em>iss,</em> the increased serum survival gene; <em>tsh,</em> the temperature sensitive hemagglutinin gene; <em>cvi,</em> the ColV immunity gene; and <em>iucC,</em> a gene of the aerobactin operon. Organisms, known for their possession or lack of these genes, were used as a source of the template DNA to develop the multiplex PCR protocol. Identity of the amplicons was confirmed by size, DNA:DNA hybridization with specific gene probes, and DNA sequencing. When the multiplex PCR protocol was used to characterize 10 <em>E. coli</em> isolates incriminated in avian colibacillosis and 10 from the feces of apparently healthy birds, nine of the isolates from apparently healthy birds contained no more than one gene, while the 10th contained all four. Also, eight of the isolates incriminated in colibacillosis contained three or more genes, while the remaining two contained two of the target genes. Interestingly, the isolates of sick birds containing only two of the targeted genes killed the least number of embryos, and the isolate of healthy birds that contained all the genes killed the most embryos among this group. These genes were not found among the non–<em>E. coli</em> isolates tested, demonstrating the procedure's specificity for <em>E. coli</em>. Overall, these results suggest that this protocol might be useful in characterization and study of avian <em>E. coli</em>.</p>
dc.description.comments <p>This article is from <em>Avian Diseases</em> 47, no. 4 (2003): 1441–1447, doi:<a href="http://dx.doi.org/10.1637/7030" target="_blank">10.1637/7030</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/vmpm_pubs/50/
dc.identifier.articleid 1049
dc.identifier.contextkey 4117707
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vmpm_pubs/50
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92356
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vmpm_pubs/50/2003_SkybergJA_CharacterizingAvianEscherichia.pdf|||Sat Jan 15 00:40:43 UTC 2022
dc.source.uri 10.1637/7030
dc.subject.disciplines Veterinary Microbiology and Immunobiology
dc.subject.disciplines Veterinary Preventive Medicine, Epidemiology, and Public Health
dc.subject.keywords Escherichia coli
dc.subject.keywords avian colibacillosis
dc.subject.keywords multiplex PCR
dc.subject.keywords virulence
dc.subject.keywords iss
dc.subject.keywords tsh
dc.subject.keywords iucC
dc.subject.keywords cvi
dc.title Characterizing Avian Escherichia coli Isolates with Multiplex Polymerase Chain Reaction
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 9e7506b4-e945-47cf-9195-e814dac6c9fd
relation.isOrgUnitOfPublication 16f8e472-b1cd-4d8f-b016-09e96dbc4d83
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