Sucrose stabilization of Respiratory Syncytial Virus (RSV) during nebulization and experimental infection

dc.contributor.author Gallup, Jack
dc.contributor.author Grosz, Drew
dc.contributor.author van Geelen, Albert
dc.contributor.author Gallup, Jack
dc.contributor.author Ackermann, Mark
dc.contributor.author Hostetter, Shannon
dc.contributor.author Derscheid, Rachel
dc.contributor.author Ackermann, Mark
dc.contributor.department Veterinary Pathology
dc.date 2018-02-16T05:32:38.000
dc.date.accessioned 2020-07-07T05:16:02Z
dc.date.available 2020-07-07T05:16:02Z
dc.date.copyright Wed Jan 01 00:00:00 UTC 2014
dc.date.issued 2014-03-18
dc.description.abstract <p>Background Respiratory syncytial virus (RSV) is a common respiratory pathogen that can cause severe pneumonia. In vivo studies of RSV can be difficult due to variation in viral infection and disease severity in some animal models. Factors that may contribute to the variation are decreases in viral titer due to preparation and storage and method of virus administration. Nebulization is one method of RSV administration that provides even distribution of virus to all lung lobes; however, the exact quantity of the virus killed by nebulization is not defined. To test the hypothesis that sucrose enhances RSV stability and infectivity, a series of in vitro experiments were conducted with RSV strain Memphis 37 stored at varying concentrations (0%, 3%, 5%, 8%, 10%, 15%, and 20%) of sucrose as a possible cryo- and nebulization protectant. The optimal in vitro concentration was then assessed in vivo in a lamb model.</p> <p>Methods Prior to titering the virus on HEp-2 cells, the various virus solutions were subjected to one freeze-thaw cycle and one nebulization cycle. Forty-eight hours after viral plating, infectious foci were detected and counted using immunofluorescent imaging. Titers were determined after freeze-thaw and after freeze-thaw followed by nebulization, then compared to the stock titers (before freezing) as well as to one another to determine the loss of infectivity. To further test this in vivo, lambs 2 to 3-days-old were infected via nebulization with RSV using inoculate containing either 20% sucrose or no sucrose followed by assessments of infection severity.</p> <p>Results Nebulization of virus in 0% sucrose resulted in a 0.580 log reduction in infectivity while virus in 20% sucrose exhibited a 0.297 log reduction. In vivo studies demonstrated that 20% sucrose enhanced RSV lesions and antigen distribution.</p> <p>Conclusions The data suggests that both nebulization and freeze-thawing of RSV in the absence of sucrose cause unacceptable losses in viral infectivity and that sucrose acts as a RSV protectant in both regards.</p>
dc.description.comments <p>This article is from <em>BMC Research Notes</em> 7 (2014): 158, doi:<a href="http://dx.doi.org/10.1186/1756-0500-7-158" target="_blank">10.1186/1756-0500-7-158</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/vpath_pubs/64/
dc.identifier.articleid 1065
dc.identifier.contextkey 7005174
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vpath_pubs/64
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92491
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vpath_pubs/64/2014_Grosz_SucroseStablilization.pdf|||Sat Jan 15 01:21:44 UTC 2022
dc.source.uri 10.1186/1756-0500-7-158
dc.subject.disciplines Animal Diseases
dc.subject.disciplines Large or Food Animal and Equine Medicine
dc.subject.disciplines Respiratory Tract Diseases
dc.subject.disciplines Veterinary Infectious Diseases
dc.subject.disciplines Veterinary Pathology and Pathobiology
dc.subject.keywords Respiratory Syncytial Virus
dc.subject.keywords RSV
dc.subject.keywords Nebulization
dc.subject.keywords Sucrose
dc.title Sucrose stabilization of Respiratory Syncytial Virus (RSV) during nebulization and experimental infection
dc.type article
dc.type.genre article
dspace.entity.type Publication
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relation.isAuthorOfPublication 86c1ed73-b60d-48ce-8f35-449bc320a693
relation.isOrgUnitOfPublication cf38d7e3-b5f8-4859-83e3-ae8fab6a4c5f
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