Flow cytometry is a promising and rapid method for differentiating between freely suspended Escherichia coli and E. coli attached to clay particles

dc.contributor.author Jarboe, Laura
dc.contributor.author Liang, Xiao
dc.contributor.author Soupir, Michelle
dc.contributor.author Soupir, Michelle
dc.contributor.author Rigby, Shawn
dc.contributor.author Jarboe, Laura
dc.contributor.author Zhang, Wei
dc.contributor.department Agricultural and Biosystems Engineering
dc.date 2018-02-16T18:02:02.000
dc.date.accessioned 2020-06-29T22:41:35Z
dc.date.available 2020-06-29T22:41:35Z
dc.date.copyright Wed Jan 01 00:00:00 UTC 2014
dc.date.embargo 2015-11-04
dc.date.issued 2014-12-01
dc.description.abstract <p><strong>Aim:</strong> A standard procedure does not exist to distinguish between attached and unattached micro-organisms. In this study, we compared two methods to quantify between Escherichia coli attached to clay particles and E. coli freely suspended in solution: flow cytometry (attachment assay and viability assay) and settling (or centrifugation followed by settling).</p> <p><strong>Methods and Results:</strong> Methods were tested using three environmental strains collected from swine facilities (A, B and C) and one purchased modified pathogenic strain (ATCC 43888); four clay particles: Hectorite, Kaolinite, Ca-Montmorillonite, Montmorillonite K-10; and a range of surface area ratios (particle surface area to E. coli surface area). When comparing the two methods, the per cent attached obtained from the flow cytometry was lower, but not significantly different from the per cent attached obtained from the settling method for all conditions except when the particle was Hectorite or Montmorillonite K-10; when the strain was C; and when the surface area ratio was below 100. Differences between the methods are likely because traditional culture-based methods cannot detect the viable but nonculturable (VBNC) population, whereas flow cytometry can detect the fraction of VBNC with intact membranes.</p> <p><strong>Conclusion:</strong> Our results indicate that flow cytometry is a rapid and culture-independent method for differentiating between attached and unattached micro-organisms.</p> <p><strong>Significance and Impact of the Study:</strong> Flow cytometry is useful for laboratory-based studies of micro-organism–particle interactions.</p>
dc.description.comments <p>This is the peer reviewed version of the following article: Liang, X., Soupir, M. L., Rigby, S., Jarboe, L. R., and Zhang, W. (2014). <em>Journal of Applied Microbiology</em> <strong>117</strong>, 1730–1739, which has been published in final form at <a href="http://dx.doi.org/%2010.1111/jam.12660" target="_blank">http://dx.doi.org/ 10.1111/jam.12660</a>. This article may be used for non-commercial purposed in accordance with <a href="http://olabout.wiley.com/WileyCDA/Section/id-820227.html" target="_blank">Wiley Terms and Conditions for Self-Archiving</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/abe_eng_pubs/650/
dc.identifier.articleid 1933
dc.identifier.contextkey 7317042
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath abe_eng_pubs/650
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/1440
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/abe_eng_pubs/650/2014_Liang_FlowCytometry.docx|||Sat Jan 15 01:23:54 UTC 2022
dc.source.bitstream archive/lib.dr.iastate.edu/abe_eng_pubs/650/auto_convert.pdf|||Sat Jan 15 01:23:56 UTC 2022
dc.source.uri 10.1111/jam.12660
dc.subject.disciplines Agriculture
dc.subject.disciplines Biological Engineering
dc.subject.disciplines Bioresource and Agricultural Engineering
dc.subject.disciplines Biostatistics
dc.subject.disciplines Biotechnology
dc.subject.keywords attachment
dc.subject.keywords clay
dc.subject.keywords E. coli
dc.subject.keywords flow cytometry
dc.subject.keywords settling
dc.title Flow cytometry is a promising and rapid method for differentiating between freely suspended Escherichia coli and E. coli attached to clay particles
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 6113d68a-37ba-4092-8ed5-44d66391fae4
relation.isAuthorOfPublication 04becbfb-7a97-4d96-a0dd-5514295530ee
relation.isOrgUnitOfPublication 8eb24241-0d92-4baf-ae75-08f716d30801
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