Detection of pseudorabies virus antibody in swine oral fluid using a serum whole-virus indirect ELISA

Date
2020-05-25
Authors
Cheng, Ting-Yu
Buckley, Alexandra
Van Geelen, Albert
Wang, Chong
Lager, Kelly
Henao-Díaz, Alexandra
Poonsuk, Korakrit
Piñeyro, Pablo
Baum, David
Ji, Ju
Wang, Chong
Main, Rodger
Zimmerman, Jeffrey
Giménez-Lirola, Luis
Giménez-Lirola, Luis
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Wang, Chong
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Giménez-Lirola, Luis
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StatisticsVeterinary Diagnostic and Production Animal MedicineVeterinary Diagnostic Laboratory
Abstract

We evaluated the detection of pseudorabies virus (PRV) antibodies in swine oral fluid. Oral fluid and serum samples were obtained from 40 pigs allocated to 4 treatment groups (10 pigs/group): negative control (NC); wild-type PRV inoculation (PRV 3CR Ossabaw; hereafter PRV); PRV vaccination (Ingelvac Aujeszky MLV; Boehringer Ingelheim; hereafter MLV); and PRV vaccination followed by PRV inoculation at 21 d post-vaccination (MLV-PRV). Using a serum PRV whole-virus indirect IgG ELISA (Idexx Laboratories) adapted to the oral fluid matrix, PRV antibody was detected in oral fluid samples from treatment groups PRV, MLV, and MLV-PRV in a pattern similar to serum. Vaccination alone produced a low oral fluid antibody response (groups MLV and MLV-PRV), but a strong anamnestic response was observed following challenge with wild-type virus (group PRV). Analyses of the oral fluid PRV indirect IgG ELISA results showed good binary diagnostic performance (area under ROC curve = 93%) and excellent assay repeatability (intra-class correlation coefficient = 99.3%). The demonstrable presence of PRV antibodies in swine oral fluids suggests the possible use of oral fluids in pseudorabies surveillance.

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This article is published as Cheng, Ting-Yu, Alexandra Buckley, Albert Van Geelen, Kelly Lager, Alexandra Henao-Díaz, Korakrit Poonsuk, Pablo Piñeyro, David Baum, Ju Ji, Chong Wang, Rodger Main, Jeffrey Zimmerman, and Luis Giménez-Lirola. "Detection of pseudorabies virus antibody in swine oral fluid using a serum whole-virus indirect ELISA." Journal of Veterinary Diagnostic Investigation (2020). DOI: 10.1177%2F1040638720924386.

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