Detection of pseudorabies virus antibody in swine oral fluid using a serum whole-virus indirect ELISA

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2020-05-25
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Cheng, Ting-Yu
Buckley, Alexandra
Van Geelen, Albert
Lager, Kelly
Henao-Díaz, Alexandra
Poonsuk, Korakrit
Piñeyro, Pablo
Baum, David
Ji, Ju
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Giménez-Lirola, Luis
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Statistics
As leaders in statistical research, collaboration, and education, the Department of Statistics at Iowa State University offers students an education like no other. We are committed to our mission of developing and applying statistical methods, and proud of our award-winning students and faculty.
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Veterinary Diagnostic and Production Animal Medicine
The mission of VDPAM is to educate current and future food animal veterinarians, population medicine scientists and stakeholders by increasing our understanding of issues that impact the health, productivity and well-being of food and fiber producing animals; developing innovative solutions for animal health and food safety; and providing the highest quality, most comprehensive clinical practice and diagnostic services. Our department is made up of highly trained specialists who span a wide range of veterinary disciplines and species interests. We have faculty of all ranks with expertise in diagnostics, medicine, surgery, pathology, microbiology, epidemiology, public health, and production medicine. Most have earned certification from specialty boards. Dozens of additional scientists and laboratory technicians support the research and service components of our department.
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Abstract

We evaluated the detection of pseudorabies virus (PRV) antibodies in swine oral fluid. Oral fluid and serum samples were obtained from 40 pigs allocated to 4 treatment groups (10 pigs/group): negative control (NC); wild-type PRV inoculation (PRV 3CR Ossabaw; hereafter PRV); PRV vaccination (Ingelvac Aujeszky MLV; Boehringer Ingelheim; hereafter MLV); and PRV vaccination followed by PRV inoculation at 21 d post-vaccination (MLV-PRV). Using a serum PRV whole-virus indirect IgG ELISA (Idexx Laboratories) adapted to the oral fluid matrix, PRV antibody was detected in oral fluid samples from treatment groups PRV, MLV, and MLV-PRV in a pattern similar to serum. Vaccination alone produced a low oral fluid antibody response (groups MLV and MLV-PRV), but a strong anamnestic response was observed following challenge with wild-type virus (group PRV). Analyses of the oral fluid PRV indirect IgG ELISA results showed good binary diagnostic performance (area under ROC curve = 93%) and excellent assay repeatability (intra-class correlation coefficient = 99.3%). The demonstrable presence of PRV antibodies in swine oral fluids suggests the possible use of oral fluids in pseudorabies surveillance.

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This article is published as Cheng, Ting-Yu, Alexandra Buckley, Albert Van Geelen, Kelly Lager, Alexandra Henao-Díaz, Korakrit Poonsuk, Pablo Piñeyro, David Baum, Ju Ji, Chong Wang, Rodger Main, Jeffrey Zimmerman, and Luis Giménez-Lirola. "Detection of pseudorabies virus antibody in swine oral fluid using a serum whole-virus indirect ELISA." Journal of Veterinary Diagnostic Investigation (2020). DOI: 10.1177%2F1040638720924386.

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