In Vitro Detection of Shiga Toxin Using Porcine Alveolar Macrophages

Date
2001-09-01
Authors
Mengeling, William
Cornick, Nancy
Vorwald, Ann
Cornick, Nancy
Lager, Kelly
Moon, Harley
Major Professor
Advisor
Committee Member
Journal Title
Journal ISSN
Volume Title
Publisher
Altmetrics
Authors
Research Projects
Organizational Units
Journal Issue
Series
Department
Veterinary Microbiology and Preventive Medicine
Abstract

Porcine alveolar macrophages were found to be highly susceptible to the cytolytic effects of a toxin (Shiga toxin [Stx]) produced by certain strains of Escherichia coli and sometimes associated with clinical disease in pigs and other animals. In comparison with the cells that are most commonly used for Stx detection and titration in vitro (namely, Vero cells), porcine alveolar macrophages appeared to be generally more sensitive and test results could be obtained in less time. Moreover, unlike Vero cells, porcine alveolar macrophages need not be continuously propagated to ensure immediate availability. They can simply be removed from a low-temperature repository, thawed, seeded, and shortly thereafter exposed to the sample in question. These characteristics suggest that porcine alveolar macrophages may be useful in developing a highly sensitive and timely diagnostic test for Stx.

Comments

This articles is from Journal of Veterinary Diagnostic Investigation 13 (2001): 421, doi:10.1177/104063870101300511.

Description
Keywords
Citation
DOI
Collections