In Vitro Detection of Shiga Toxin Using Porcine Alveolar Macrophages Mengeling, William Cornick, Nancy Vorwald, Ann Cornick, Nancy Lager, Kelly Moon, Harley
dc.contributor.department Veterinary Microbiology and Preventive Medicine 2018-02-17T09:56:52.000 2020-07-07T05:14:23Z 2020-07-07T05:14:23Z 2001-09-01
dc.description.abstract <p>Porcine alveolar macrophages were found to be highly susceptible to the cytolytic effects of a toxin (Shiga toxin [Stx]) produced by certain strains of <em>Escherichia coli</em> and sometimes associated with clinical disease in pigs and other animals. In comparison with the cells that are most commonly used for Stx detection and titration in vitro (namely, Vero cells), porcine alveolar macrophages appeared to be generally more sensitive and test results could be obtained in less time. Moreover, unlike Vero cells, porcine alveolar macrophages need not be continuously propagated to ensure immediate availability. They can simply be removed from a low-temperature repository, thawed, seeded, and shortly thereafter exposed to the sample in question. These characteristics suggest that porcine alveolar macrophages may be useful in developing a highly sensitive and timely diagnostic test for Stx.</p>
dc.description.comments <p>This articles is from <em>Journal of Veterinary Diagnostic Investigation</em> 13 (2001): 421, doi:<a href="" target="_blank">10.1177/104063870101300511</a>.</p>
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dc.identifier archive/
dc.identifier.articleid 1105
dc.identifier.contextkey 7984107
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vmpm_pubs/118
dc.language.iso en
dc.source.bitstream archive/|||Fri Jan 14 18:58:30 UTC 2022
dc.source.uri 10.1177/104063870101300511
dc.subject.disciplines Veterinary Medicine
dc.subject.disciplines Veterinary Microbiology and Immunobiology
dc.subject.disciplines Veterinary Toxicology and Pharmacology
dc.title In Vitro Detection of Shiga Toxin Using Porcine Alveolar Macrophages
dc.type article
dc.type.genre article
dspace.entity.type Publication
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