In Vitro Detection of Shiga Toxin Using Porcine Alveolar Macrophages

dc.contributor.author Mengeling, William
dc.contributor.author Cornick, Nancy
dc.contributor.author Vorwald, Ann
dc.contributor.author Cornick, Nancy
dc.contributor.author Lager, Kelly
dc.contributor.author Moon, Harley
dc.contributor.department Veterinary Microbiology and Preventive Medicine
dc.date 2018-02-17T09:56:52.000
dc.date.accessioned 2020-07-07T05:14:23Z
dc.date.available 2020-07-07T05:14:23Z
dc.date.issued 2001-09-01
dc.description.abstract <p>Porcine alveolar macrophages were found to be highly susceptible to the cytolytic effects of a toxin (Shiga toxin [Stx]) produced by certain strains of <em>Escherichia coli</em> and sometimes associated with clinical disease in pigs and other animals. In comparison with the cells that are most commonly used for Stx detection and titration in vitro (namely, Vero cells), porcine alveolar macrophages appeared to be generally more sensitive and test results could be obtained in less time. Moreover, unlike Vero cells, porcine alveolar macrophages need not be continuously propagated to ensure immediate availability. They can simply be removed from a low-temperature repository, thawed, seeded, and shortly thereafter exposed to the sample in question. These characteristics suggest that porcine alveolar macrophages may be useful in developing a highly sensitive and timely diagnostic test for Stx.</p>
dc.description.comments <p>This articles is from <em>Journal of Veterinary Diagnostic Investigation</em> 13 (2001): 421, doi:<a href="http://dx.doi.org/10.1177/104063870101300511" target="_blank">10.1177/104063870101300511</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/vmpm_pubs/118/
dc.identifier.articleid 1105
dc.identifier.contextkey 7984107
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vmpm_pubs/118
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92219
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vmpm_pubs/118/2001_Cornick_InVitroDetection.pdf|||Fri Jan 14 18:58:30 UTC 2022
dc.source.uri 10.1177/104063870101300511
dc.subject.disciplines Veterinary Medicine
dc.subject.disciplines Veterinary Microbiology and Immunobiology
dc.subject.disciplines Veterinary Toxicology and Pharmacology
dc.title In Vitro Detection of Shiga Toxin Using Porcine Alveolar Macrophages
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication a89b8fad-4329-4bb1-bbcd-c1d3b324f0f7
relation.isOrgUnitOfPublication 16f8e472-b1cd-4d8f-b016-09e96dbc4d83
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