Gene expression patterns during somatic embryo development and germination in maize Hi II cultures

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dc.contributor.author Che, Ping
dc.contributor.author Love, Tanzy
dc.contributor.author Carriquiry, Alicia
dc.contributor.author Frame, Bronwyn
dc.contributor.author Wang, Kan
dc.contributor.author Carriquiry, Alicia
dc.contributor.author Howell, Stephen
dc.contributor.department Statistics
dc.date 2018-02-16T18:50:42.000
dc.date.accessioned 2020-07-02T06:56:10Z
dc.date.available 2020-07-02T06:56:10Z
dc.date.issued 2004-11-01
dc.description.abstract <p>Gene expression changes associated with embryogenic callus formation and with somatic embryo maturation and germination were examined in a regenerationproficient hybrid line of Zea mays, Hi II. 12,060 element maize cDNA microarrays were used to generate gene expression profiles from embryogenic calli induced to undergo embryo maturation and germination. No statistically significant gene expression changes were detected in comparing embryogenic with total callus. On the other hand, over 1,000 genes showed significant time variation during somatic embryo development. In general, a substantial number of genes were downregulated during embryo maturation, largely histone and ribosomal protein genes, which may result from a slow down in cell proliferation and growth during embryo maturation. The expression of these genes dramatically recovered at germination. Other genes upregulated during embryo maturation included genes encoding hydrolytic enzymes (nucleases, glucosidases and proteases) and a few storage genes (zein and caleosin), which are good candidates for developmental marker genes. Germination is accompanied by the upregulation of a number of stress response and membrane transporter genes, and, as expected, greening is associated with the upregulation of many genes encoding photosynthetic and chloroplast components. Thus, some, but not all genes, typically associated with zygotic embryogenesis are significantly up or downregulated during somatic embryogenesis in Hi II maize line regeneration.</p>
dc.description.comments <p>This preprint was published as Ping Che, Tanzy Love, Bronwyn Frame, Kan Wang, Alicia Carriquiry and Stephen Howell, "Gene Expression Patterns During Somatic Embryo Development and Germination in Maize Hi II Callus Cultures", <em>Plant Molecular Biology</em> (2006): 1-14, doi: <a href="http://dx.doi.org/10.1007/s11103-006-9013-2" target="_blank">10.1007/s11103-006-9013-2</a>.</p>
dc.identifier archive/lib.dr.iastate.edu/stat_las_preprints/38/
dc.identifier.articleid 1036
dc.identifier.contextkey 7327495
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath stat_las_preprints/38
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/90330
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/stat_las_preprints/38/2004_CarriquiryAL_GeneExpressionPatterns.pdf|||Fri Jan 14 23:52:06 UTC 2022
dc.source.uri 10.1007/s11103-006-9013-2
dc.subject.disciplines Statistics and Probability
dc.subject.keywords Plant Sciences Institute
dc.subject.keywords Center for Plant Transformation
dc.subject.keywords Agronomy Department
dc.title Gene expression patterns during somatic embryo development and germination in maize Hi II cultures
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 6ddd5891-2ad0-4a93-89e5-8c35c28b0de4
relation.isOrgUnitOfPublication 264904d9-9e66-4169-8e11-034e537ddbca
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