Validation of candidate genes putatively associated with resistance to SCMV and MDMV in maize (Zea mays L.) by expression profiling

Date
2009-02-02
Authors
Użarowska, Anna
Dionisio, Giuseppe
Lubberstedt, Thomas
Sarholz, Barbara
Piepho, Hans-Peter
Xu, Mingliang
Ingvardsen, Christina
Wenzel, Gerhard
Lubberstedt, Thomas
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Agronomy
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Agronomy
Abstract

Background

The potyviruses sugarcane mosaic virus (SCMV) and maize dwarf mosaic virus (MDMV) are major pathogens of maize worldwide. Two loci, Scmv1 and Scmv2, have ealier been shown to confer complete resistance to SCMV. Custom-made microarrays containing previously identified SCMV resistance candidate genes and resistance gene analogs were utilised to investigate and validate gene expression and expression patterns of isogenic lines under pathogen infection in order to obtain information about the molecular mechanisms involved in maize-potyvirus interactions.

Results

By employing time course microarray experiments we identified 68 significantly differentially expressed sequences within the different time points. The majority of differentially expressed genes differed between the near-isogenic line carrying Scmv1 resistance locus at chromosome 6 and the other isogenic lines. Most differentially expressed genes in the SCMV experiment (75%) were identified one hour after virus inoculation, and about one quarter at multiple time points. Furthermore, most of the identified mapped genes were localised outside the Scmv QTL regions. Annotation revealed differential expression of promising pathogenesis-related candidate genes, validated by qRT-PCR, coding for metallothionein-like protein, S-adenosylmethionine synthetase, germin-like protein or 26S ribosomal RNA.

Conclusion

Our study identified putative candidate genes and gene expression patterns related to resistance to SCMV. Moreover, our findings support the effectiveness and reliability of the combination of different expression profiling approaches for the identification and validation of candidate genes. Genes identified in this study represent possible future targets for manipulation of SCMV resistance in maize.

Comments

This article is published as Użarowska, Anna, Giuseppe Dionisio, Barbara Sarholz, Hans-Peter Piepho, Mingliang Xu, Christina Rønn Ingvardsen, Gerhard Wenzel, and Thomas Lübberstedt. "Validation of candidate genes putatively associated with resistance to SCMV and MDMV in maize (Zea mays L.) by expression profiling." BMC Plant Biology 9, no. 1 (2009): 15. doi: 10.1186/1471-2229-9-15. Posted with permission.

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