Roles of lipooligosaccharide and capsular polysaccharide in antimicrobial resistance and natural transformation of Campylobacter jejuni

dc.contributor.author Jeon, Byeonghwa
dc.contributor.author Muraoka, Wayne
dc.contributor.author Zhang, Qijing
dc.contributor.author Scupham, Alexandra
dc.contributor.author Zhang, Qijing
dc.contributor.department Veterinary Microbiology and Preventive Medicine
dc.date 2018-02-18T23:07:16.000
dc.date.accessioned 2020-07-07T05:14:36Z
dc.date.available 2020-07-07T05:14:36Z
dc.date.issued 2009-03-01
dc.description.abstract <p>Objectives</p> <p>This study is aimed to determine the role of capsular polysaccharide (CPS) and lipooligosaccharide (LOS) in modulating antimicrobial resistance and natural transformation of <em>Campylobacter jejuni</em>, an important food-borne human pathogen.</p> <p>Methods</p> <p>A series of <em>C</em>. <em>jejuni</em> mutants, which are defective in either CPS or LOS or both, were constructed. The antimicrobial susceptibility, bacterial surface hydrophobicity, natural transformation frequency and DNA binding and uptake were measured and compared between the mutants and the wild-type strain.</p> <p>Results</p> <p>Truncation of LOS greatly reduced (8-fold) the intrinsic resistance of <em>C</em>. <em>jejuni</em> to erythromycin, a key antibiotic used for treating human campylobacteriosis, while the loss of CPS did not result in significant changes in the susceptibility to antimicrobial agents. Notably, mutation of LOS also significantly increased (>16-fold) the susceptibility to erythromycin in <em>C</em>. <em>jejuni</em> mutants carrying the A2074G mutation in 23S rRNA. The increased susceptibility to erythromycin in the LOS mutant was probably due to enhanced permeability to this antibiotic, because the LOS mutation rendered the surface of <em>C</em>. <em>jejuni</em> more hydrophobic. Loss of CPS and truncation of LOS increased the transformation frequency by 4- and 25-fold, respectively, and mutation of both CPS and LOS resulted in a 97-fold increase in the transformation frequency. Consistent with the increased transformation frequencies, the CPS and LOS mutants showed enhanced rates of DNA uptake.</p> <p>Conclusions</p> <p>These results demonstrate that the surface polysaccharides in <em>C</em>. <em>jejuni</em> contribute to the resistance to erythromycin, a clinically important antibiotic, but restrict natural transformation.</p>
dc.description.comments <p>This article is published as Jeon, Byeonghwa, Wayne Muraoka, Alexandra Scupham, and Qijing Zhang. "Roles of lipooligosaccharide and capsular polysaccharide in antimicrobial resistance and natural transformation of Campylobacter jejuni." Journal of antimicrobial chemotherapy 63, no. 3 (2009): 462-468. doi: <a href="https://doi.org/10.1093/jac/dkn529">10.1093/jac/dkn529</a>. Posted with permission.</p>
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dc.identifier archive/lib.dr.iastate.edu/vmpm_pubs/149/
dc.identifier.articleid 1148
dc.identifier.contextkey 10786527
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vmpm_pubs/149
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92253
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vmpm_pubs/149/2009_Zhang_RolesLipooligosaccharide.pdf|||Fri Jan 14 20:28:26 UTC 2022
dc.source.uri 10.1093/jac/dkn529
dc.subject.disciplines Genetics and Genomics
dc.subject.disciplines Veterinary Microbiology and Immunobiology
dc.subject.disciplines Veterinary Pathology and Pathobiology
dc.subject.disciplines Veterinary Preventive Medicine, Epidemiology, and Public Health
dc.subject.keywords food safety
dc.subject.keywords macrolides
dc.subject.keywords competence
dc.subject.keywords drug susceptibility
dc.title Roles of lipooligosaccharide and capsular polysaccharide in antimicrobial resistance and natural transformation of Campylobacter jejuni
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 1c6a5dfc-c604-457f-85be-122910db782e
relation.isOrgUnitOfPublication 16f8e472-b1cd-4d8f-b016-09e96dbc4d83
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