Tear Fluid Pharmacokinetics Following Oral Prednisone Administration in Dogs With and Without Conjunctivitis

dc.contributor.author Smith, Joe
dc.contributor.author Sebbag, Lionel
dc.contributor.author Mochel, Jonathan
dc.contributor.author Sebbag, Lionel
dc.contributor.author Yan, Yuqi
dc.contributor.author Smith, Joe
dc.contributor.author Allbaugh, Rachel
dc.contributor.author Wulf, Larry
dc.contributor.author Allbaugh, Rachel
dc.contributor.author Mochel, Jonathan
dc.contributor.department Biomedical Sciences
dc.contributor.department Veterinary Clinical Sciences
dc.contributor.department Veterinary Diagnostic and Production Animal Medicine
dc.contributor.department Veterinary Diagnostic Laboratory
dc.date 2019-07-08T05:16:41.000
dc.date.accessioned 2020-07-07T05:12:22Z
dc.date.available 2020-07-07T05:12:22Z
dc.date.copyright Tue Jan 01 00:00:00 UTC 2019
dc.date.issued 2019-05-09
dc.description.abstract <p><em>Purpose:</em> To describe the pharmacokinetics (PK) of prednisone and prednisolone in tear fluid of dogs receiving oral prednisone at anti-inflammatory to immunosuppressive doses and to assess the impact of induced conjunctivitis on lacrimal drug levels.</p> <p><em>Methods:</em> Six healthy Beagle dogs were administered 4 courses of prednisone at 0.5, 1.0, 2.0, and 4.0 mg/kg given orally once a day for 5 days. At steady state, topical histamine was applied to induce mild (1 mg/mL) or severe (375 mg/mL) conjunctivitis in 1 eye of each dog and tear samples were collected from both eyes at selected times. Prednisone and prednisolone were quantified in tears by liquid chromatography-mass spectrometry.</p> <p><em>Results:</em> Lacrimal prednisone and prednisolone concentrations ranged from 2 to 523 ng/mL and 5 to 191 ng/mL, respectively. Drug concentrations were overall greater in dogs receiving higher doses of prednisone, but were not correlated with tear flow rate. Eyes with conjunctivitis often had larger amounts of prednisone and prednisolone in tear fluid compared to control eyes (up to +64%), but differences were not statistically significant. Significantly greater, but clinically insignificant, levels of prednisolone were found in eyes with severe versus mild conjunctivitis for oral prednisone doses ≥1.0 mg/kg.</p> <p><em>Conclusions:</em> Disruption of the blood–tear barrier with conjunctivitis did not significantly affect drug levels in tears. Based on drug PK in tears, oral prednisone is likely safe for the management of reflex uveitis and ocular surface diseases. However, further prospective trials using systemic corticotherapy in diseased animals are warranted to confirm findings from this preclinical study.</p>
dc.description.comments <p>This is a manuscript of an article published as Sebbag, Lionel, Yuqi Yan, Joe S. Smith, Rachel A. Allbaugh, Larry W. Wulf, and Jonathan P. Mochel. "Tear Fluid Pharmacokinetics Following Oral Prednisone Administration in Dogs With and Without Conjunctivitis." <em>Journal of Ocular Pharmacology and Therapeutics</em> (2019). DOI: <a href="http://dx.doi.org/10.1089/jop.2019.0020" target="_blank">10.1089/jop.2019.0020</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/vcs_pubs/31/
dc.identifier.articleid 1030
dc.identifier.contextkey 14495187
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vcs_pubs/31
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/91910
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vcs_pubs/31/2019_AllbaughRachel_TearFluid.pdf|||Fri Jan 14 23:31:05 UTC 2022
dc.source.uri 10.1089/jop.2019.0020
dc.subject.disciplines Eye Diseases
dc.subject.disciplines Pharmacology
dc.subject.disciplines Small or Companion Animal Medicine
dc.subject.disciplines Veterinary Infectious Diseases
dc.subject.keywords pharmacokinetics
dc.subject.keywords prednisone
dc.subject.keywords prednisolone
dc.subject.keywords tear film
dc.subject.keywords canine
dc.subject.keywords blood–tear barrier
dc.title Tear Fluid Pharmacokinetics Following Oral Prednisone Administration in Dogs With and Without Conjunctivitis
dc.type article
dc.type.genre article
dspace.entity.type Publication
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