Maize Transgenes Containing Zein Promoters are Regulated by opaque2

dc.contributor.author Schroder, Megan
dc.contributor.author Floyd, Brice
dc.contributor.author Scott, Marvin
dc.contributor.author Scott, M. Paul
dc.contributor.department Agronomy
dc.contributor.department Plant Biology
dc.contributor.department Genetics
dc.date 2018-02-18T14:48:11.000
dc.date.accessioned 2020-06-29T23:02:56Z
dc.date.available 2020-06-29T23:02:56Z
dc.date.issued 2011-01-01
dc.description.abstract <p>Understanding epistatic interactions between transgenes and native genes is critical for predicting the response of transgenes to different genetic backgrounds and environments. Our objective was to determine if predicted epistatic interactions occur between maize (<em>Zea mays</em> L.) transgenes carrying zein promoters and <em>opaque2</em>, a transcriptional regulator of zein genes. Expression of the transgenes was significantly decreased in kernels containing the <em>opaque2</em> mutation. Native zein proteins in these kernels were reduced as well. Thus, transgene expression mirrored expression of the native gene that contributed the promoter of the transgene. This work demonstrates that information about native gene expression may be useful for predicting transgene expression, reducing the risk of unintended consequences of transgene expression.</p>
dc.description.comments <p>This article is published as Schroder, Megan N., Brice E. Floyd, and M. Paul Scott. "Maize Transgenes Containing Zein Promoters are Regulated by." <em>Crop science</em> 51, no. 6 (2011): 2716-2720, doi: <a href="http://dx.doi.org/10.2135/cropsci2011.03.0168" target="_blank">10.2135/cropsci2011.03.0168</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/agron_pubs/148/
dc.identifier.articleid 1155
dc.identifier.contextkey 10425222
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath agron_pubs/148
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/4475
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/agron_pubs/148/2011_Scott_MaizeTransgenes.pdf|||Fri Jan 14 20:26:45 UTC 2022
dc.source.uri 10.2135/cropsci2011.03.0168
dc.subject.disciplines Agricultural Science
dc.subject.disciplines Agronomy and Crop Sciences
dc.subject.disciplines Plant Breeding and Genetics
dc.title Maize Transgenes Containing Zein Promoters are Regulated by opaque2
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 97acee5f-1291-4c27-8929-a8e1617c411d
relation.isOrgUnitOfPublication fdd5c06c-bdbe-469c-a38e-51e664fece7a
File
Original bundle
Now showing 1 - 1 of 1
Name:
2011_Scott_MaizeTransgenes.pdf
Size:
544.65 KB
Format:
Adobe Portable Document Format
Description:
Collections