Advancements of spontaneous haploid genome doubling (SHGD) via haploid male fertility (HMF) in Maize (Zea mays L.) for doubled haploid (DH) breeding
Date
2023-12
Authors
Foster, Tyler Lee
Major Professor
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Lübberstedt, Thomas
Scott, Paul
Yu, Jianming
Hufford, Matthew
Wang, Lizhi
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Abstract
Doubled haploid (DH) technology has innovated maize breeding. With DH technology, completely homozygous lines are developed with a considerably shorter breeding cycle than traditional inbred development. Traditional DH line development routinely uses chemical doubling agents, such as colchicine, to induce genome doubling. Alternatively, plant breeders can use a genetic mechanism, known as spontaneous haploid genome doubling (SHGD). Unfortunately, haploid male fertility (HMF) has been a major bottleneck of SHGD for DH production in maize. In this study, a protocol for evaluating SHGD via HMF was developed. In addition, a bi-parental population was created to fine map a large-effect quantitative trait locus (QTL), qshgd1, where results indicated the QTL lies within a 25 megabase (Mb) region on chromosome 5. In addition, 286 sweet corn genotypes were evaluated for their HMF ability. From this study, 11 genotypes with elevated levels of HMF were found. Along with these findings, ten single nucleotide polymorphisms (SNPs) within eight candidate genes were discovered to be associated with HMF. Finally, 423 sweet corn genotypes were evaluated for their ability to express the anthocyanin marker, R1-navajo, where no clear tend between subpopulations was identified – signaling expression of this marker could be controlled by different QTL.
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dissertation