Using CRISPR-Cas9 to Create a Null Allele of Outsiders in D. Melanogaster

dc.contributor.author Anderson, Jasmine
dc.contributor.department Genetics, Development and Cell Biology
dc.date 2018-02-14T09:56:10.000
dc.date.accessioned 2020-07-07T05:10:21Z
dc.date.available 2020-07-07T05:10:21Z
dc.date.issued 2014-04-15
dc.description.abstract <p>Programmed cell death (PCD) is a biological process that shapes human development. Yet, cancer cells are insusceptible to this process leading to the proliferation of tumors. Research on PCD can produce cancer therapies which increase tumor susceptibility to PCD for tumor eradication.</p> <p>The exact mechanisms of PCD are currently unknown. My research aims to uncover the role of the gene outsiders in the scheme of PCD in Drosophila melanogaster (fruit fly) embryos. During embryogenesis, Drosophila germ cells travel across the embryo to the gonads for proper development. Mutants with the outsiders gene respond less to PCD resulting in the correct number of germ cells in the gonads, but an excess outlying the peripherals.</p> <p>To decipher the mechanisms involved in PCD, outsiders will be excised from the genome using the CRISPR-Cas9 genetic engineering technique. This knock-out phenotype will provide insight on the network of PCD for human health applications.</p>
dc.identifier archive/lib.dr.iastate.edu/undergradresearch_symposium/2014/presentations/12/
dc.identifier.articleid 1011
dc.identifier.contextkey 5914570
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath undergradresearch_symposium/2014/presentations/12
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/91573
dc.relation.ispartofseries Symposium on Undergraduate Research and Creative Expression
dc.source.bitstream archive/lib.dr.iastate.edu/undergradresearch_symposium/2014/presentations/12/AndersonJasmine.pdf|||Fri Jan 14 19:04:19 UTC 2022
dc.subject.disciplines Genetics
dc.title Using CRISPR-Cas9 to Create a Null Allele of Outsiders in D. Melanogaster
dc.type event
dc.type.genre article
dspace.entity.type Publication
relation.isOrgUnitOfPublication 9e603b30-6443-4b8e-aff5-57de4a7e4cb2
relation.isSeriesOfPublication 6730f354-97b8-4408-bad3-7e5c3b2fca9d
thesis.degree.discipline Genetics
File
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
AndersonJasmine.pdf
Size:
161.38 KB
Format:
Adobe Portable Document Format
Description: