Effect of Recombinant Human Cytokines on Porcine Neutrophil Function

Date
1993-06-01
Authors
Coe, Nancy
Frank, Dagmar
Roth, James
Roth, James
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Roth, James
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Veterinary Microbiology and Preventive Medicine
Abstract

The activity of four recombinant human cytokines on porcine neutrophils was evaluated. Porcine neutrophils were treated with varying doses of recombinant human tumor necrosis factor-alpha (rHu-TNF), interferon-gamma (rHu-IFN), interleukin-8 (rHu-IL-8), or granulocyte-macrophage colony-stimulating factor (rHu-GM-CSF). The function of treated neutrophils was compared with that of non-treated controls in the following assays: antibody-independent neutrophil cytotoxicity (AINC), antibody-dependent cell-mediated cytotoxicity (ADCC), iodination, Staphylococcus aureus ingestion, cytochrome C reduction, random migration, and chemotaxis. Treatment with rHu-TNF produced significant (P < 0.05) depression of neutrophil random migration (2.5, 25, and 250 ng ml−1 rHu-TNF) and iodination (250 ng ml−1) and a near significant (P = 0.08) depression in ADCC (250 ng ml−1). Treatment with 25 000 U ml−1 of rHu-IFN caused a significant increase in AINC. At lower doses of rHu-IFN, there was a trend (0.05 < P ≤ 0.08) toward depression of AINC (250 U ml−1) and ADCC (25 U ml−1) and enhancement of iodination (250 U ml−1). Treatment with 50 ng ml−1 of rHu-IL-8 caused a near significant increase (P= 0.06)M in AINC. There were no significant differences noted when porcine neutrophils were treated with rHu-GM-CSF (2.5–2500 U ml−1). No synergism was noted between rHu-TNF and rHu-IFN.

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This article is from Veterinary Immunology and Immunopathology 37 (1993): 39, doi:10.1016/0165-2427(93)90014-U.

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