Effect of Recombinant Human Cytokines on Porcine Neutrophil Function
dc.contributor.author | Coe, Nancy | |
dc.contributor.author | Frank, Dagmar | |
dc.contributor.author | Roth, James | |
dc.contributor.author | Roth, James | |
dc.contributor.department | Veterinary Microbiology and Preventive Medicine | |
dc.date | 2018-02-17T04:43:04.000 | |
dc.date.accessioned | 2020-07-07T05:15:18Z | |
dc.date.available | 2020-07-07T05:15:18Z | |
dc.date.issued | 1993-06-01 | |
dc.description.abstract | <p>The activity of four recombinant human cytokines on porcine neutrophils was evaluated. Porcine neutrophils were treated with varying doses of recombinant human tumor necrosis factor-alpha (rHu-TNF), interferon-gamma (rHu-IFN), interleukin-8 (rHu-IL-8), or granulocyte-macrophage colony-stimulating factor (rHu-GM-CSF). The function of treated neutrophils was compared with that of non-treated controls in the following assays: antibody-independent neutrophil cytotoxicity (AINC), antibody-dependent cell-mediated cytotoxicity (ADCC), iodination, <em>Staphylococcus aureus</em> ingestion, cytochrome C reduction, random migration, and chemotaxis. Treatment with rHu-TNF produced significant (<em>P</em> < 0.05) depression of neutrophil random migration (2.5, 25, and 250 ng ml<sup>−1</sup> rHu-TNF) and iodination (250 ng ml<sup>−1</sup>) and a near significant (<em>P</em> = 0.08) depression in ADCC (250 ng ml<sup>−1</sup>). Treatment with 25 000 U ml<sup>−1</sup> of rHu-IFN caused a significant increase in AINC. At lower doses of rHu-IFN, there was a trend (0.05 < <em>P</em> ≤ 0.08) toward depression of AINC (250 U ml<sup>−1</sup>) and ADCC (25 U ml<sup>−1</sup>) and enhancement of iodination (250 U ml<sup>−1</sup>). Treatment with 50 ng ml<sup>−1</sup> of rHu-IL-8 caused a near significant increase (<em>P</em>= 0.06)<em>M</em> in AINC. There were no significant differences noted when porcine neutrophils were treated with rHu-GM-CSF (2.5–2500 U ml<sup>−1</sup>). No synergism was noted between rHu-TNF and rHu-IFN.</p> | |
dc.description.comments | <p>This article is from <em>Veterinary Immunology and Immunopathology</em> 37 (1993): 39, doi:<a href="http://dx.doi.org/10.1016/0165-2427(93)90014-U" id="x-ddDoi" target="_blank">10.1016/0165-2427(93)90014-U</a>.</p> | |
dc.format.mimetype | application/pdf | |
dc.identifier | archive/lib.dr.iastate.edu/vmpm_pubs/61/ | |
dc.identifier.articleid | 1060 | |
dc.identifier.contextkey | 7790268 | |
dc.identifier.s3bucket | isulib-bepress-aws-west | |
dc.identifier.submissionpath | vmpm_pubs/61 | |
dc.identifier.uri | https://dr.lib.iastate.edu/handle/20.500.12876/92368 | |
dc.language.iso | en | |
dc.source.bitstream | archive/lib.dr.iastate.edu/vmpm_pubs/61/1993_Roth_EffectRecombinant.pdf|||Sat Jan 15 01:16:05 UTC 2022 | |
dc.source.uri | 10.1016/0165-2427(93)90014-U | |
dc.subject.disciplines | Veterinary Microbiology and Immunobiology | |
dc.subject.disciplines | Veterinary Preventive Medicine, Epidemiology, and Public Health | |
dc.title | Effect of Recombinant Human Cytokines on Porcine Neutrophil Function | |
dc.type | article | |
dc.type.genre | article | |
dspace.entity.type | Publication | |
relation.isAuthorOfPublication | 909dd0b2-ec0a-41e2-b4e0-9e5ff76b7622 | |
relation.isOrgUnitOfPublication | 16f8e472-b1cd-4d8f-b016-09e96dbc4d83 |
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