Effect of Recombinant Human Cytokines on Porcine Neutrophil Function

dc.contributor.author Coe, Nancy
dc.contributor.author Frank, Dagmar
dc.contributor.author Roth, James
dc.contributor.author Roth, James
dc.contributor.department Veterinary Microbiology and Preventive Medicine
dc.date 2018-02-17T04:43:04.000
dc.date.accessioned 2020-07-07T05:15:18Z
dc.date.available 2020-07-07T05:15:18Z
dc.date.issued 1993-06-01
dc.description.abstract <p>The activity of four recombinant human cytokines on porcine neutrophils was evaluated. Porcine neutrophils were treated with varying doses of recombinant human tumor necrosis factor-alpha (rHu-TNF), interferon-gamma (rHu-IFN), interleukin-8 (rHu-IL-8), or granulocyte-macrophage colony-stimulating factor (rHu-GM-CSF). The function of treated neutrophils was compared with that of non-treated controls in the following assays: antibody-independent neutrophil cytotoxicity (AINC), antibody-dependent cell-mediated cytotoxicity (ADCC), iodination, <em>Staphylococcus aureus</em> ingestion, cytochrome C reduction, random migration, and chemotaxis. Treatment with rHu-TNF produced significant (<em>P</em> < 0.05) depression of neutrophil random migration (2.5, 25, and 250 ng ml<sup>−1</sup> rHu-TNF) and iodination (250 ng ml<sup>−1</sup>) and a near significant (<em>P</em> = 0.08) depression in ADCC (250 ng ml<sup>−1</sup>). Treatment with 25 000 U ml<sup>−1</sup> of rHu-IFN caused a significant increase in AINC. At lower doses of rHu-IFN, there was a trend (0.05 < <em>P</em> ≤ 0.08) toward depression of AINC (250 U ml<sup>−1</sup>) and ADCC (25 U ml<sup>−1</sup>) and enhancement of iodination (250 U ml<sup>−1</sup>). Treatment with 50 ng ml<sup>−1</sup> of rHu-IL-8 caused a near significant increase (<em>P</em>= 0.06)<em>M</em> in AINC. There were no significant differences noted when porcine neutrophils were treated with rHu-GM-CSF (2.5–2500 U ml<sup>−1</sup>). No synergism was noted between rHu-TNF and rHu-IFN.</p>
dc.description.comments <p>This article is from <em>Veterinary Immunology and Immunopathology</em> 37 (1993): 39, doi:<a href="http://dx.doi.org/10.1016/0165-2427(93)90014-U" id="x-ddDoi" target="_blank">10.1016/0165-2427(93)90014-U</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/vmpm_pubs/61/
dc.identifier.articleid 1060
dc.identifier.contextkey 7790268
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vmpm_pubs/61
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92368
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vmpm_pubs/61/1993_Roth_EffectRecombinant.pdf|||Sat Jan 15 01:16:05 UTC 2022
dc.source.uri 10.1016/0165-2427(93)90014-U
dc.subject.disciplines Veterinary Microbiology and Immunobiology
dc.subject.disciplines Veterinary Preventive Medicine, Epidemiology, and Public Health
dc.title Effect of Recombinant Human Cytokines on Porcine Neutrophil Function
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 909dd0b2-ec0a-41e2-b4e0-9e5ff76b7622
relation.isOrgUnitOfPublication 16f8e472-b1cd-4d8f-b016-09e96dbc4d83
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