Using Arabidopsis Mesophyll Protoplasts to Study Unfolded Protein Response Signaling
| dc.contributor.author | Bao, Yan | |
| dc.contributor.author | Bassham, Diane | |
| dc.contributor.author | Bassham, Diane | |
| dc.contributor.department | Genetics, Development and Cell Biology | |
| dc.date | 2019-05-21T20:55:43.000 | |
| dc.date.accessioned | 2020-06-30T04:02:00Z | |
| dc.date.available | 2020-06-30T04:02:00Z | |
| dc.date.copyright | Mon Jan 01 00:00:00 UTC 2018 | |
| dc.date.issued | 2018-12-05 | |
| dc.description.abstract | <p>Various environmental stresses or artificial reagents can trigger unfolded protein accumulation in the endoplasmic reticulum (ER) due to the folding capacity of the ER being exceeded. This is termed ER stress, and triggers the unfolded protein response (UPR). Assays for activation of the UPR in plants include Tunicamycin (Tm)- or dithiothreitol (DTT)-mediated root growth inhibition, analysis of splicing of the UPR-responsive transcription factor bZIP60 (basic Leucine Zipper Domain 60), and upregulation of relevant UPR genes. We provide here a quick and robust method to detect UPR signaling in Arabidopsis thaliana protoplasts. This assay can also be applied to other plant species for which protoplasts can be isolated.</p> | |
| dc.description.comments | <p>This article is published Bao, Yan, and Diane C. Bassham. "Using Arabidopsis Mesophyll Protoplasts to Study Unfolded Protein Response Signaling." <em>Bio-protocol</em> 8, no. 23 (2018) e3101. doi: <a href="https://doi.org/10.21769/BioProtoc.3101">10.21769/BioProtoc.3101</a>. Posted with permission.</p> | |
| dc.format.mimetype | application/pdf | |
| dc.identifier | archive/lib.dr.iastate.edu/gdcb_las_pubs/222/ | |
| dc.identifier.articleid | 1226 | |
| dc.identifier.contextkey | 14202314 | |
| dc.identifier.s3bucket | isulib-bepress-aws-west | |
| dc.identifier.submissionpath | gdcb_las_pubs/222 | |
| dc.identifier.uri | https://dr.lib.iastate.edu/handle/20.500.12876/37897 | |
| dc.language.iso | en | |
| dc.source.bitstream | archive/lib.dr.iastate.edu/gdcb_las_pubs/222/0-Permission_from_Bio_protocol.pdf|||Fri Jan 14 22:42:36 UTC 2022 | |
| dc.source.bitstream | archive/lib.dr.iastate.edu/gdcb_las_pubs/222/2018_Bassham_UsingArabidopsis.pdf|||Fri Jan 14 22:42:38 UTC 2022 | |
| dc.source.uri | 10.21769/BioProtoc.3101 | |
| dc.subject.disciplines | Cell Biology | |
| dc.subject.disciplines | Developmental Biology | |
| dc.subject.disciplines | Plant Biology | |
| dc.subject.keywords | Arabidopsis | |
| dc.subject.keywords | bZIP60 splicing | |
| dc.subject.keywords | DTT | |
| dc.subject.keywords | ER stress | |
| dc.subject.keywords | Protoplasts | |
| dc.subject.keywords | Tm | |
| dc.subject.keywords | UPR | |
| dc.title | Using Arabidopsis Mesophyll Protoplasts to Study Unfolded Protein Response Signaling | |
| dc.type | article | |
| dc.type.genre | article | |
| dspace.entity.type | Publication | |
| relation.isAuthorOfPublication | 2d25a522-f3af-4bb3-9221-363ff3668fe0 | |
| relation.isOrgUnitOfPublication | 9e603b30-6443-4b8e-aff5-57de4a7e4cb2 |