Certain Listeria monocytogenes plasmids contribute to increased UVC ultraviolet light stress

dc.contributor.author Anast, Justin
dc.contributor.author Schmitz-Esser, Stephan
dc.contributor.department Animal Science
dc.contributor.department Microbiology
dc.date.accessioned 2022-02-21T13:08:34Z
dc.date.available 2022-02-21T13:08:34Z
dc.date.issued 2021-09
dc.description.abstract Listeria monocytogenes is the causative agent of the highly fatal foodborne disease listeriosis and can persist in food production environments. Recent research highlights the involvement of L. monocytogenes plasmids in different stress response mechanisms, which contribute to its survival in food production facilities. Ultraviolet (UV) light in the UVC spectrum (200–280 nm) is used in food production to control microbial contamination. Although plasmid-encoded UV resistance mechanisms have been described in other bacteria, no research indicates that L. monocytogenes plasmids contribute to the UV stress response. The plasmids of L. monocytogenes strains 6179, 4KSM and R479a are genetically distinct and were utilized to study the roles of plasmids in the UV response. Wild-type and plasmid-cured variant cells were grown to logarithmic or late-stationary phase, plated on agar plates and exposed to UVC for 60 or 90 s, and colony-forming units (CFUs) were determined. CFUs of 6179 and 4KSM, bearing pLM6179 and p4KSM, respectively, were significantly (P-value < 0.05) higher than those of the plasmid-cured strains in both logarithmic and stationary phases. No difference in survival was observed for the R479a strain. Our data show for the first time that certain L. monocytogenes plasmids contribute to the survival of UVC light stress.
dc.description.comments This article is published as Anast, Justin M., and Stephan Schmitz-Esser. "Certain Listeria monocytogenes plasmids contribute to increased UVC ultraviolet light stress." FEMS Microbiology Letters 368, no. 17 (2021): fnab123. doi:10.1093/femsle/fnab123. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/ywAbnn4v
dc.language.iso en
dc.publisher © The Author(s) 2021
dc.source.uri https://doi.org/10.1093/femsle/fnab123 *
dc.subject.disciplines DegreeDisciplines::Life Sciences::Animal Sciences
dc.subject.disciplines DegreeDisciplines::Life Sciences::Food Science::Food Microbiology
dc.subject.keywords Listeria monocytogenes
dc.subject.keywords foodborne illness
dc.subject.keywords plasmid
dc.subject.keywords ultraviolet light
dc.subject.keywords food production stress
dc.subject.keywords persistence
dc.title Certain Listeria monocytogenes plasmids contribute to increased UVC ultraviolet light stress
dc.type Article
dspace.entity.type Publication
relation.isAuthorOfPublication 247d6138-be53-44be-9e88-220424e98124
relation.isAuthorOfPublication 247d6138-be53-44be-9e88-220424e98124
relation.isOrgUnitOfPublication 85ecce08-311a-441b-9c4d-ee2a3569506f
relation.isOrgUnitOfPublication 76818945-1d8c-461a-bbac-df615c052c9e
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