Collecting oral fluid samples from due-to-wean litters

dc.contributor.author Almeida, Marcelo
dc.contributor.author Rotto, H.
dc.contributor.author Linhares, Daniel
dc.contributor.author Schneider, P.
dc.contributor.author Robb, C.
dc.contributor.author Zimmerman, Jeffrey
dc.contributor.author Holtkamp, Derald
dc.contributor.author Rademacher, Christopher
dc.contributor.author Linhares, Daniel
dc.contributor.department Veterinary Diagnostic and Production Animal Medicine
dc.date 2019-11-08T14:56:26.000
dc.date.accessioned 2020-07-07T05:13:00Z
dc.date.available 2020-07-07T05:13:00Z
dc.date.copyright Tue Jan 01 00:00:00 UTC 2019
dc.date.embargo 2020-11-04
dc.date.issued 2019-11-04
dc.description.abstract <p>Oral fluids are a common diagnostic sample in group-housed nursery, grow-finish, and adult swine. Although oral fluids from due-to-wean litters could be a valuable tool in monitoring pathogens and predicting the health status of pig populations post-weaning, it is generally not done because of inconsistent success in sample collection. The objective of this study was to determine the optimum procedure for collecting oral fluid samples from due-to-wean litters. Successful collection of oral fluids from due-to-wean litters using "Litter Oral Fluid" (LOF) or "Family Oral Fluid" (FOF) sampling techniques were compared in 4 phases involving 920 attempts to collect oral fluids. Phase 1 testing showed that prior exposure to a rope improved the success rates of both LOF (33.4%) and FOF (16.4%) techniques. Phase 2 determined that longer access to the rope (4 hours vs 30 minutes) did not improve the success rate for either LOF or FOF. Phase 3 evaluated the effect of attractants and found that one (Baby Pig Restart®) improved the success rate when used with the FOF technique. Phase 4 compared the success rates of "optimized LOF" (litters previously trained) vs "optimized FOF" (litter previously trained and rope treated with Baby Pig Restart®) vs standard FOF. No difference was found between the FOF-based techniques, but both were superior to the "optimized LOF" technique. Thus, FOF-based procedures provided a significantly higher probability of collecting oral fluids from due-to-wean litters (mean success rate 84.9%, range 70% to 92%) when compared to LOF-based methods (mean success rate 24.1%, range 16.5% to 32.2%).</p>
dc.description.comments <p>This is a manuscript of an article published as Almeida, M. N., H. Rotto, P. Schneider, C. Robb, J. J. Zimmerman, D. J. Holtkamp, C. J. Rademacher, and D. C. L. Linhares. "Collecting oral fluid samples from due-to-wean litters." <em>Preventive Veterinary Medicine</em> (2019): 104810. DOI: <a href="http://dx.doi.org/10.1016/j.prevetmed.2019.104810" target="_blank">10.1016/j.prevetmed.2019.104810</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/vdpam_pubs/162/
dc.identifier.articleid 1166
dc.identifier.contextkey 15733534
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vdpam_pubs/162
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92007
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vdpam_pubs/162/2019_LinharesDaniel_CollectingOral.pdf|||Fri Jan 14 20:56:37 UTC 2022
dc.source.uri 10.1016/j.prevetmed.2019.104810
dc.subject.disciplines Large or Food Animal and Equine Medicine
dc.subject.disciplines Veterinary Preventive Medicine, Epidemiology, and Public Health
dc.subject.keywords Swine
dc.subject.keywords Oral fluids
dc.subject.keywords Surveillance
dc.title Collecting oral fluid samples from due-to-wean litters
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 3ce0db9e-1f42-4d29-b389-2364b3470254
relation.isOrgUnitOfPublication 5ab07352-4171-4f53-bbd7-ac5d616f7aa8
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