Ontogeny of the Maize Shoot Apical Meristem

Date
2012-08-01
Authors
Takacs, Elizabeth
Nettleton, Dan
Li, Jie
Du, Chuanlong
Ponnala, Lalit
Janick-Buckner, Diane
Yu, Jianming
Muehlbauer, Gary
Schnable, Patrick
Timmermans, Marja
Sun, Qi
Nettleton, Dan
Scanlon, Michael
Major Professor
Advisor
Committee Member
Journal Title
Journal ISSN
Volume Title
Publisher
Altmetrics
Authors
Research Projects
Organizational Units
Statistics
Organizational Unit
Agronomy
Organizational Unit
Journal Issue
Series
Department
StatisticsAgronomy
Abstract

The maize (Zea mays) shoot apical meristem (SAM) arises early in embryogenesis and functions during stem cell maintenance and organogenesis to generate all the aboveground organs of the plant. Despite its integral role in maize shoot development, little is known about the molecular mechanisms of SAM initiation. Laser microdissection of apical domains from developing maize embryos and seedlings was combined with RNA sequencing for transcriptomic analyses of SAMontogeny. Molecular markers of key events during maize embryogenesis are described, and comprehensive transcriptional data from six stages in maize shoot development are generated. Transcriptomic profiling before and after SAM initiation indicates that organogenesis precedes stem cell maintenance in maize; analyses of the first three lateral organs elaborated from maize embryos provides insight into their homology and to the identity of the single maize cotyledon. Compared with the newly initiated SAM, the mature SAM is enriched for transcripts that function in transcriptional regulation, hormonal signaling, and transport. Comparisons of shoot meristems initiating juvenile leaves, adult leaves, and husk leaves illustrate differences in phase-specific (juvenile versus adult) and meristem-specific (SAM versus lateral meristem) transcript accumulation during maize shoot development. This study provides insight into the molecular genetics of SAMinitiation and function in maize.

Comments

This article is published as Takacs, Elizabeth M., Jie Li, Chuanlong Du, Lalit Ponnala, Diane Janick-Buckner, Jianming Yu, Gary J. Muehlbauer et al. "Ontogeny of the maize shoot apical meristem." The Plant Cell 24, no. 8 (2012): 3219-3234. doi: 10.1105/tpc.112.099614. Copyright American Society of Plant Biologists. Posted with permission.

Description
Keywords
Citation
DOI
Collections