QTL Mapping of Low-Temperature Germination Ability in the Maize IBM Syn4 RIL Population

dc.contributor.author Hu, Shuaidong
dc.contributor.author Lubberstedt, Thomas
dc.contributor.author Lubberstedt, Thomas
dc.contributor.author Guangwu Zhao, Guangwu
dc.contributor.author Lee, Michael
dc.contributor.department Agronomy
dc.date 2018-02-19T00:19:55.000
dc.date.accessioned 2020-06-29T23:03:54Z
dc.date.available 2020-06-29T23:03:54Z
dc.date.copyright Fri Jan 01 00:00:00 UTC 2016
dc.date.issued 2016-03-31
dc.description.abstract <p>Low temperature is the primary factor to affect maize sowing in early spring. It is, therefore, vital for maize breeding programs to improve tolerance to low temperatures at seed germination stage. However, little is known about maize QTL involved in low-temperature germination ability. 243 lines of the intermated B73×Mo17 (IBM) Syn4 recombinant inbred line (RIL) population was used for QTL analysis of low-temperature germination ability. There were significant differences in germination-related traits under both conditions of low temperature (12°C/16h, 18°C/8h) and optimum temperature (28°C/24h) between the parental lines. Only three QTL were identified for controlling optimum-temperature germination rate. Six QTL controlling low-temperature germination rate were detected on chromosome 4, 5, 6, 7 and 9, and contribution rate of single QTL explained between 3.39%~11.29%. In addition, six QTL controlling low-temperature primary root length were detected in chromosome 4, 5, 6, and 9, and the contribution rate of single QTL explained between 3.96%~8.41%. Four pairs of QTL were located at the same chromosome position and together controlled germination rate and primary root length under low temperature condition. The nearest markers apart from the corresponding QTL (only 0.01 cM) were umc1303 (265.1 cM) on chromosome 4, umc1 (246.4 cM) on chromosome 5, umc62 (459.1 cM) on chromosome 6, bnl14.28a (477.4 cM) on chromosome 9, respectively. A total of 3155 candidate genes were extracted from nine separate intervals based on the Maize Genetics and Genomics Database (http://www.maizegdb.org). Five candidate genes were selected for analysis as candidates putatively affecting seed germination and seedling growth at low temperature. The results provided a basis for further fine mapping, molecular marker assisted breeding and functional study of cold-tolerance at the stage of seed germination in maize.</p>
dc.description.comments <p>This article is published as Hu, Shuaidong, Thomas Lübberstedt, Guangwu Zhao, and Michael Lee. "QTL mapping of low-temperature germination ability in the maize IBM Syn4 RIL population." PloS one 11, no. 3 (2016): e0152795. doi:<a href="https://doi.org/10.1371/journal.pone.0152795">10.1371/journal.pone.0152795</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/agron_pubs/273/
dc.identifier.articleid 1264
dc.identifier.contextkey 10950467
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath agron_pubs/273
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/4613
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/agron_pubs/273/2016_Lubberstedt_QTLMapping.PDF|||Fri Jan 14 23:06:56 UTC 2022
dc.source.uri 10.1371/journal.pone.0152795
dc.subject.disciplines Agronomy and Crop Sciences
dc.title QTL Mapping of Low-Temperature Germination Ability in the Maize IBM Syn4 RIL Population
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 4e4330cd-db15-4ac5-8924-41119139cf32
relation.isOrgUnitOfPublication fdd5c06c-bdbe-469c-a38e-51e664fece7a
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