Danofloxacin Treatment Alters the Diversity and Resistome Profile of Gut Microbiota in Calves

dc.contributor.author Beyi, Ashenafi Feyisa
dc.contributor.author Brito-Goulart, Debora
dc.contributor.author Hawbecker, Tyler
dc.contributor.author Slagel, Clare
dc.contributor.author Ruddell, Brandon
dc.contributor.author Hassall, Alan
dc.contributor.author Dewell, Renee
dc.contributor.author Dewell, Grant
dc.contributor.author Sahin, Orhan
dc.contributor.department Veterinary Microbiology and Preventive Medicine
dc.contributor.department Veterinary Diagnostic and Production Animal Medicine
dc.contributor.department Center for Food Security and Public Health
dc.contributor.other National Institute of Antimicrobial Resistance Research and Education
dc.date.accessioned 2021-12-17T19:12:56Z
dc.date.available 2021-12-17T19:12:56Z
dc.date.issued 2021-09-24
dc.description.abstract Fluoroquinolones, such as danofloxacin, are used to control bovine respiratory disease complex in beef cattle; however, little is known about their effects on gut microbiota and resistome. The objectives were to evaluate the effect of subcutaneously administered danofloxacin on gut microbiota and resistome, and the composition of Campylobacter in calves. Twenty calves were injected with a single dose of danofloxacin, and ten calves were kept as a control. The effects of danofloxacin on microbiota and the resistome were assessed using 16S rRNA sequencing, quantitative real-time PCR, and metagenomic Hi-C ProxiMeta. Alpha and beta diversities were significantly different (p < 0.05) between pre-and post-treatment samples, and the compositions of several bacterial taxa shifted. The patterns of association between the compositions of Campylobacter and other genera were affected by danofloxacin. Antimicrobial resistance genes (ARGs) conferring resistance to five antibiotics were identified with their respective reservoirs. Following the treatment, some ARGs (e.g., ant9, tet40, tetW) increased in frequencies and host ranges, suggesting initiation of horizontal gene transfer, and new ARGs (aac6, ermF, tetL, tetX) were detected in the post-treatment samples. In conclusion, danofloxacin induced alterations of gut microbiota and selection and enrichment of resistance genes even against antibiotics that are unrelated to danofloxacin.
dc.description.comments This article is published as Beyi, Ashenafi Feyisa, Debora Brito-Goulart, Tyler Hawbecker, Clare Slagel, Brandon Ruddell, Alan Hassall, Renee Dewell et al. "Danofloxacin Treatment Alters the Diversity and Resistome Profile of Gut Microbiota in Calves." Microorganisms 9, no. 10 (2021): 2023. DOI: 10.3390/microorganisms9102023. Copyright: © 2021 by the authors. Creative Commons Attribution (CC BY) license. Posted with permission.
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/8zn7YK7w
dc.language.iso en_US
dc.publisher MDPI
dc.source.uri https://doi.org/10.3390/microorganisms9102023 *
dc.subject antimicrobial resistance genes (ARGs)
dc.subject bovine calves
dc.subject bovine respiratory disease
dc.subject Campylobacter
dc.subject danofloxacin/fluoroquinolones
dc.subject fecal microbiota
dc.subject resistome
dc.subject.disciplines DegreeDisciplines::Medicine and Health Sciences::Veterinary Medicine::Large or Food Animal and Equine Medicine
dc.title Danofloxacin Treatment Alters the Diversity and Resistome Profile of Gut Microbiota in Calves
dc.type Article
dspace.entity.type Publication
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