Porcine epidemic diarrhea virus (PEDV) detection and antibody response in commercial growing pigs

dc.contributor.author Bjustrom-Kraft, Jordan
dc.contributor.author Woodard, Katie
dc.contributor.author Gimenez-Lirola, Luis
dc.contributor.author Wang, Chong
dc.contributor.author Rotolo, Marisa
dc.contributor.author Wang, Chong
dc.contributor.author Sun, Yaxuan
dc.contributor.author Lasley, Peter
dc.contributor.author Zhang, Jianqiang
dc.contributor.author Baum, David
dc.contributor.author Gauger, Phillip
dc.contributor.author Main, Rodger
dc.contributor.author Zimmerman, Jeffrey
dc.contributor.department Statistics
dc.contributor.department Veterinary Diagnostic and Production Animal Medicine
dc.date 2018-06-18T20:32:15.000
dc.date.accessioned 2020-07-07T05:12:38Z
dc.date.available 2020-07-07T05:12:38Z
dc.date.copyright Fri Jan 01 00:00:00 UTC 2016
dc.date.issued 2016-06-10
dc.description.abstract <p>Background: Longitudinal samples from two production sites were used to (1) describe the pattern of PEDV shedding (rRT-PCR) in individual rectal swabs, pen fecal samples, and pen oral fluids (OF); (2) describe the kinetics of PEDV antibody by ELISA (IgA, IgG) testing of pig serum and pen oral fluid samples; and (3) establish cutoffs and performance estimates for PEDV WV ELISAs (IgA, IgG). Site One was PEDV positive; Site Two was PEDV negative. On Site One, pen samples (feces and oral fluids) and pig samples (rectal swabs and sera) were collected both before and after the population was exposed to PEDV.</p> <p>Results: On Site Two, pen oral fluid samples and individual pig serum samples were negative for both PEDV antibody and nucleic acid. On Site One, PEDV was detected by rRT-PCR at 6 days post exposure (DPE) in all sample types. The last rRT-PCR positives were detected in rectal swabs and oral fluids on 69 DPE. IgG and IgA were detected in oral fluids and serum samples by 13 DPE. Analysis of the PEDV serum IgG WV ELISA data showed that a sample-to-positive (S/P) cutoff of ≥ 0.80 provided a diagnostic sensitivity of 0.87 (95 % CI: 0.82, 0.91) and specificity of 0.99 (95 % CI: 0.98, 1.00). Serum IgG results declined slowly over the monitoring period, with 60 % of serum samples positive (S/P ≥ 0.80) at the final sampling on 111 DPE. Analysis of the PEDV oral fluid IgA WV ELISA found that a cutoff of S/P ≥ 0.80 provided a diagnostic sensitivity of 1.00 (95 % CI: 0.92, 1.00) and a diagnostic specificity of 1.00 (95 % CI: 0.99, 1.00). The oral fluid IgA response increased through 96 DPE and began to decline at the last sampling on 111 DPE.</p> <p>Conclusions: This study showed that oral fluid-based testing could provide an easy and “animal-friendly” approach to sample collection for nucleic acid and/or antibody-based surveillance of PEDV in swine populations.</p>
dc.description.comments <p>This article is published as Bjustrom-Kraft, Jordan, Katie Woodard, Luis Giménez-Lirola, Marisa Rotolo, Chong Wang, Yaxuan Sun, Peter Lasley et al. "Porcine epidemic diarrhea virus (PEDV) detection and antibody response in commercial growing pigs." <em>BMC veterinary research</em> 12 (2016): 99. doi: <a href="https://doi.org/10.1186/s12917-016-0725-5" target="_blank">10.1186/s12917-016-0725-5</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/vdpam_pubs/110/
dc.identifier.articleid 1115
dc.identifier.contextkey 12333857
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vdpam_pubs/110
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/91951
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vdpam_pubs/110/2016_Wang_PorcineEpidemic.pdf|||Fri Jan 14 18:40:12 UTC 2022
dc.source.uri 10.1186/s12917-016-0725-5
dc.subject.disciplines Large or Food Animal and Equine Medicine
dc.subject.disciplines Statistics and Probability
dc.subject.disciplines Veterinary Infectious Diseases
dc.subject.disciplines Veterinary Preventive Medicine, Epidemiology, and Public Health
dc.subject.keywords PEDV
dc.subject.keywords Virus shedding
dc.subject.keywords Antibody kinetics
dc.subject.keywords Oral fluids
dc.subject.keywords Surveillance
dc.subject.keywords IgG
dc.subject.keywords IgA
dc.title Porcine epidemic diarrhea virus (PEDV) detection and antibody response in commercial growing pigs
dc.type article
dc.type.genre article
dspace.entity.type Publication
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relation.isOrgUnitOfPublication 5ab07352-4171-4f53-bbd7-ac5d616f7aa8
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