FOXO Regulates Neuromuscular Junction Homeostasis During Drosophila Aging Birnbaum, Allison Sodders, Maggie Bouska, Mark Chang, Kai Kang, Ping McNeill, Elizabeth Bai, Hua
dc.contributor.department Genetics, Development and Cell Biology
dc.contributor.department Food Science and Human Nutrition 2022-03-24T19:10:32Z 2022-03-24T19:10:32Z 2021-01
dc.description.abstract The transcription factor foxo is a known regulator of lifespan extension and tissue homeostasis. It has been linked to the maintenance of neuronal processes across many species and has been shown to promote youthful characteristics by regulating cytoskeletal flexibility and synaptic plasticity at the neuromuscular junction (NMJ). However, the role of foxo in aging neuromuscular junction function has yet to be determined. We profiled adult Drosophila foxo- null mutant abdominal ventral longitudinal muscles and found that young mutants exhibited morphological profiles similar to those of aged wild-type flies, such as larger bouton areas and shorter terminal branches. We also observed changes to the axonal cytoskeleton and an accumulation of late endosomes in foxo null mutants and motor neuron-specific foxo knockdown flies, similar to those of aged wild-types. Motor neuron-specific overexpression of foxo can delay age-dependent changes to NMJ morphology, suggesting foxo is responsible for maintaining NMJ integrity during aging. Through genetic screening, we identify several downstream factors mediated through foxo-regulated NMJ homeostasis, including genes involved in the MAPK pathway. Interestingly, the phosphorylation of p38 was increased in the motor neuron-specific foxo knockdown flies, suggesting foxo acts as a suppressor of p38/MAPK activation. Our work reveals that foxo is a key regulator for NMJ homeostasis, and it may maintain NMJ integrity by repressing MAPK signaling.
dc.description.comments This article is published as Birnbaum A, Sodders M, Bouska M, Chang K, Kang P, McNeill E and Bai H (2021) FOXO Regulates Neuromuscular Junction Homeostasis During Drosophila Aging. Front. Aging Neurosci. 12:567861. doi: 10.3389/fnagi.2020.567861. Posted with permission. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
dc.language.iso en
dc.publisher © 2021 Birnbaum, Sodders, Bouska, Chang, Kang, McNeill and Bai
dc.source.uri *
dc.subject.keywords FOXO
dc.subject.keywords NMJ
dc.subject.keywords MAPK
dc.subject.keywords p38
dc.subject.keywords Rab7
dc.subject.keywords late endosome
dc.subject.keywords aging
dc.title FOXO Regulates Neuromuscular Junction Homeostasis During Drosophila Aging
dc.type Article
dspace.entity.type Publication
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