Immunosensing Platforms Using Spontaneously Adsorbed Antibody Fragments on Gold

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Date
2000-01-01
Authors
O'Brien, Janese
Jones, Vivian
Porter, Marc
Mosher, Curtis
Henderson, Eric
Henderson, Eric
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Ames Laboratory
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Zoology and Genetics
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Chemistry
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Ames LaboratoryZoology and GeneticsChemistry
Abstract

This paper describes the construction and characterization of miniaturized antigenic immunosurfaces composed of spontaneously adsorbed Fab‘-SH fragments on gold. Rabbit Fab‘-SH fragments contain a free sulfhydryl group that forms a thiolate bond with a gold substrate as detailed by X-ray photoelectron spectroscopy. This approach creates surfaces of higher epitope density, a factor critical to the early detection of disease, than surfaces composed of adsorbed whole molecule IgG on gold. The viability and specificity of antigenic Fab‘-SH immunosurfaces is demonstrated using atomic force microscopy and confocal fluorescence microscopy, and possible explanations for the larger epitope density are discussed.

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Reprinted (adapted) with permission from Immunosensing Platforms Using Spontaneously Adsorbed Antibody Fragments on Gold. Janese C. O'Brien,Vivian W. Jones, and, Marc D. Porter, Curtis L. Mosher and Eric Henderson. Analytical Chemistry 2000 72 (4), 703-710. DOI: 10.1021/ac990581e. Copyright 2000 American Chemical Society.

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