Polo-Like Kinase 3 Appears Dispensable for Normal Retinal Development Despite Robust Embryonic Expression

dc.contributor.author Goetz, Jillian
dc.contributor.author Laboissonniere, Lauren
dc.contributor.author Trimarchi, Jeffrey
dc.contributor.author Wester, Andrea
dc.contributor.author Lynch, Madison
dc.contributor.author Trimarchi, Jeffrey
dc.contributor.department Genetics, Development and Cell Biology
dc.date 2018-02-18T04:44:47.000
dc.date.accessioned 2020-06-30T04:02:51Z
dc.date.available 2020-06-30T04:02:51Z
dc.date.copyright Fri Jan 01 00:00:00 UTC 2016
dc.date.issued 2016-01-01
dc.description.abstract <p>During retinogenesis seven different cell types are generated in distinct yet overlapping timepoints from a population of retinal progenitor cells. Previously, we performed single cell transcriptome analyses of retinal progenitor cells to identify candidate genes that may play roles in the generation of early-born retinal neurons. Based on its expression pattern in subsets of early retinal cells, polo-like kinase 3 (Plk3) was identified as one such candidate gene. Further characterization of Plk3 expression by <em>in situ</em> hybridization revealed that this gene is expressed as cells exit the cell cycle. We obtained a Plk3 deficient mouse and investigated changes in the retina’s morphology and transcriptome through immunohistochemistry, <em>in situ</em> hybridization and gene expression profiling. These experiments have been performed initially on adult mice and subsequently extended throughout retinal development. Although morphological studies revealed no consistent changes in retinogenesis upon Plk3 loss, microarray profiling revealed potential candidate genes altered in Plk3-KO mice. Further studies will be necessary to understand the connection between these changes in gene expression and the loss of a protein kinase such as Plk3.</p>
dc.description.comments <p>This article is from <em>PloS ONE </em>11 (2016): e0150878, doi: <a href="http://dx.doi.org/10.1371/journal.pone.0150878">10.1371/journal.pone.0150878</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/gdcb_las_pubs/86/
dc.identifier.articleid 1085
dc.identifier.contextkey 9722797
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath gdcb_las_pubs/86
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/37999
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/gdcb_las_pubs/86/2016_Trimarchi_PoloLike.PDF|||Sat Jan 15 02:14:05 UTC 2022
dc.source.uri 10.1371/journal.pone.0150878
dc.subject.disciplines Cell Biology
dc.subject.disciplines Developmental Biology
dc.subject.disciplines Genetics
dc.title Polo-Like Kinase 3 Appears Dispensable for Normal Retinal Development Despite Robust Embryonic Expression
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 6e9f21d0-82ad-4661-a8f7-7634953c036b
relation.isOrgUnitOfPublication 9e603b30-6443-4b8e-aff5-57de4a7e4cb2
Original bundle
Now showing 1 - 1 of 1
30.29 MB
Adobe Portable Document Format