Effect of surface morphologies and chemistry of paper on deposited collagen

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Boddupalli, Anuraag
Boyer, Andrea
Orondo, Millicent
Bloch, Jean-Francis
Bratlie, Kaitlin
Thuo, Martin
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Bratlie, Kaitlin
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Materials Science and Engineering
Materials engineers create new materials and improve existing materials. Everything is limited by the materials that are used to produce it. Materials engineers understand the relationship between the properties of a material and its internal structure — from the macro level down to the atomic level. The better the materials, the better the end result — it’s as simple as that.
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Ames National LaboratoryElectrical and Computer EngineeringMaterials Science and EngineeringCenter for Crops Utilization ResearchChemical and Biological Engineering

Paper-based platforms for biological studies have received significant attention given that cellulose is ubiquitous, biocompatible, and can be readily organized into tunable fibrous structures. In the latter form, effect of complexity in surface morphologies (roughness, porosity and fiber organization) on cell-substrate interaction has not been thoroughly explored. We infer that altering the properties of a fibrous material should lead to significant changes in cellular microenvironment and direct the deposition of structurally analogous extracellular matrix (fiber-fiber templating) like collagen. Here, we elucidate the effect of varying paper roughness and surface chemistry on NIH/3T3 fibroblasts via organization of excreted collagen. Collagen intensity was found to increase linearly with paper porosity, indicating a 3D culture platform. The intensity, however, decays over time due to biodegradation of the substrate. Stability can be improved by introducing fluorinated alkyl silanes to yield hydrophobic paper. This process concomitantly transforms the substrate to a 2D-like scaffold where collagen is predominantly assembled on the surface, thus changing the cellular microenvironment. Altering surface energy also led to fluctuations in collagen intensity and organization over time for smooth (calendered) paper substrates. We infer that the increased roughness improves collagen adsorption through capillary driven petal effect. In general, the influence of the substrate simultaneously affects its ability to host collagen and guide orientation. These findings offer insights into the effects of secondary structures and chemistry of fibrous polymeric materials on cell culture, which we propose as vital parameters when using paper-based platforms.


This is a manuscript of an article published as Chang, Boyce S., Anuraag Boddupalli, Andrea F. Boyer, Millicent Orondo, Jean-Francis Bloch, Kaitlin M. Bratlie, and Martin M. Thuo. "Effect of surface morphologies and chemistry of paper on deposited collagen." Applied Surface Science 484 (2019): 461-469. DOI: 10.1016/j.apsusc.2019.04.131. Posted with permission.

Tue Jan 01 00:00:00 UTC 2019